Anti-Vimentin antibody [RV202] - Cytoskeleton Marker

• 产品名称

  Anti-Vimentin抗体[RV202] - Cytoskeleton Marker
  参阅全部 Vimentin 一抗

• 描述

  小鼠单克隆抗体[RV202] to Vimentin - Cytoskeleton Marker

• 宿主

  Mouse

• 特异性

  This antibody reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.

• 经测试应用

  适用于: IHC-Fr, WB, IHC-P, Flow Cyt (Intra), ICC/IFmore details

• 种属反应性

  与反应: Mouse, Rat, Human, Pig, Zebrafish
  

• 免疫原

  Recombinant full length protein corresponding to Bovine Vimentin. RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a vimentin extract of bovine lens.
  Database link: P08670

• 阳性对照

• IHC-Fr: Human lung, Ed18 rat, pig colon and zebrafish embryo. ICC/IF: Human colon, rat brain, SK-N-SH, human pancreatic adenocarcinoma cells and HAP1 cells. WB: NIH-3T3 and normal human dermal fibroblasts cell extracts. IHC-P: Human placenta, human small intestine, human spleen, human tonsillar lymphoma, human colon tissue, human fetal kidney tissue, dog soft tissue sarcoma tissue. Flow Cyt (intra): HeLa cells.

• 常规说明

  Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

  RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

   

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituent: PBS

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  RV202

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab8978于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 7431 Human

• Entrez Gene: 22352 Mouse

• Entrez Gene: 100522394 Pig

• Entrez Gene: 81818 Rat

• Omim: 193060 Human

• SwissProt: P08670 Human

• SwissProt: P20152 Mouse

• SwissProt: P02543 Pig

• SwissProt: P31000 Rat

• Unigene: 455493 Human

• Unigene: 691131 Human

• Unigene: 268000 Mouse

• Unigene: 2710 Rat

• 
  

• 形式

  Vimentin is found in connective tissue and in the cytoskeleton.

• 别名

• HEL113 antibody

• VIM antibody

• VIME_HUMAN antibody

• Vimentin antibody

• 
  

• CTRCT30 antibody

• Epididymis luminal protein 113 antibody

• FLJ36605 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)This image is courtesy of an anonymous Abreview

  Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of 10% normal buffered formalin-fixed pig lung tissue. Stained with ab8978 at 1/300 dilution. Secondary antibody used was CY3 conjugated donkey anti-mouse secondary antibody at 1/200 dilution. Blocking was done with 5% serum for 1 hour at 22°C. The sample was incubated with the primary antibody for 16 hours at 4°C. Antigen retrieval method was heat mediated, TE pH9.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

  ab8978 staining Vimentin in wild-type HAP1 cells (top panel) and VIM knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab8978 at 1μg/ml and ab202272 (Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 594)) at 1/250 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with ab150117 (Goat secondary antibody to Mouse IgG (Alexa Fluor® 488)) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Western blot - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

  All lanes : Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)
  
  Lane 1 : NIH3T3 cell lysate
  Lane 2 : Normal human dermal fibroblasts cell extracts.
  
  Predicted band size: 53 kDa
  Observed band size: 57 kDawhy is the actual band size different from the predicted?
  
  

• 

  Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

  Immunohistochemistry analysis of frozen section of pig colon labeling Vimentin with ab8978, showing positive staining in connective tissue cells and no reactivity in epithelial cells.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

  Immunohistochemistry analysis of formalin fixed, paraffin embedded section of human placenta labeling Vimentin with ab8978, showing positive staining in connective tissue cells and no reactivity in epithelial cells.

• 

  Flow Cytometry (Intracellular) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

  Overlay histogram showing HeLa cells stained with ab8978 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8978, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This anti-Vimentin antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)Rauschert, I. et al PLoS One. 2017 Apr 19;12(4):e0175953. doi: 10.1371/journal.pone.0175953. eCollection 2017 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Silencing of Lamin A/C in unmethylated neuroblastoma cells induces changes in different cytoskeletal components

  Immunofluorescence staining showing changes in SK-N-SH-lamin-A/C-shRNA compared with SK-N-SH-scramble-shRNA in (A) β-actin filaments, (B) F-actin filaments. (C) Vimentin filaments, and (D) α-tubulin. Lamin A/C is shown in green; β-actin, F-actin, vimentin, and α-tubulin are shown in red.DNA is stained in blue (DAPI). Scale bar, 10μM.

  Vimentin is detected using ab8978 at 1/100 dilution. Neuroblastoma cells were fixed with 4% paraformaldehyde and permeabilized using 0.5% Triton X-100.

  (From Figure 5C of Rauschert et al)

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

  Immunohistochemistry analysis of formalin fixed, paraffin embedded section of human small intestine labeling Vimentin with ab8978; showing positive staining in connective tissue cells and no reactivity in epithelial cells.

• 

  Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

  Immunohistochemistry analysis of frozen section of pig colon labeling Vimentin with ab8978, showing positive staining in connective tissue cells and no reactivity in epithelial cells. Nuclear staining with DAPI.

• 

  Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

  Immunohistochemistry analysis of frozen section of zebrafish embryo labeling Vimentin with ab8978.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

  Immunohistochemistry analysis of formalin fixed, paraffin embedded section of human spleen labeling Vimentin with ab8978, showing positive staining in connective tissue cells and lymphoid cells.

• 

  Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

  Immunohistochemistry analysis of frozen section of zebrafish embryo labeling Vimentin with ab8978.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

  Paraffin-embedded human colon tissue stained for Vimentin using ab8978 at 1/100 dilution in immunohistochemical analysis.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)This image is courtesy of an anonymous abreview.

  ab8978 staining Vimentin in human fetal kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with CAS-Block for 1 hour at 25°C; antigen retrieval was by heat mediation using OmniPrep (pH 9). Samples were incubated with primary antibody (1/500) for 1 hour at 25°C. An Alexa Fluor® 555-conjugated Donkey polyclonal (1/200) was used as the secondary antibody.

  See Abreview

• 

  Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

  Immunofluorescence staining images of 9 day old zebrafish embryos. ab8978 reacts with in connective tissue cells and bloodvessels. Frozen sample treated with Acetone:Methanol 1:1, antibody diluted 1/100 and incubated for 45 minutes at room temperature.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)This image is courtesy of an Abreview submitted by Dr J. Chai

  ab8978 staining Vimentin in human colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized in 0.1% Triton and blocked with 0.25% serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in antibody diluent) for 2 hours at 28°C. ab6785 Goat polyclonal anti-Mouse IgG - H&L (FITC) (1/800) was used as the secondary antibody. Nuclei were counterstained with propidium iodide.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)This image is courtesy of an anonymous abreview.

  ab8978 staining Vimentin in dog soft tissue sarcoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a Tris/EDTA pH9 buffer. Samples were incubated with primary antibody (1/100 in TBS) for 18 hours at 20°C. A Alexa Fluor® 647-conjugated Goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody.

  See Abreview

• 

  Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)Image courtesy of an AbReview submitted by Dr Ruma Raha-Chowdhury

  IHC-Fr image of Ed18 rat stained with ab8978. Fresh frozen sections were incubated in 10% normal donkey serum in 0.1% PBS- and 0.3% triton X100  for 1h to permeabilise the tissues and block non-specific protein-protein interactions. The sectons were then incubated with the ab8978 (1µg/ml) and ferroportin overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 568 (red) donkey anti-mouse at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Vimentin expressed in the gut muscles.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)

  Immunohistochemistry analysis of formalin fixed, paraffin embedded section of human tonsillar lymphoma labeling Vimentin with ab8978.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)This image is courtesy of Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.

  IHC-FoFr image of vimentin staining on rat injured cortical sections using ab8978 (1:500). The brain was perfusion fixed using 4% PFA and the sections were permeabilized using 0.1% TritonX in 0.1% PBS. The sections were then blocked using 10% donkey serum for 1 hour at 24°C. ab8978 was diluted 1:500 and incubated with sections for 24 hours using 4°C. The secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)This image is courtesy of an Abreview submitted by Jennifer Dembinski

  ab8978 staining vimentin in human pancreatic adenocarcinoma cells by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.2% Triton X prior to blocking in 3% BSA for 30 minutes at 24°C. The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 21°C. Alexa fluor® 488 mouse polyclonal to mouse Ig, diluted 1/300 was used as the secondary antibody.

  See Abreview

• 

  Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV202] - Cytoskeleton Marker (ab8978)This image is courtesy of an anonymous abreview.

  ab8978 staining Vimentin in human lung tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and blocking with 5% commercially available blocking agent was performed at 370C  for 15 minutes. The sample was incubated with primary antibody (1/250) at 370C for 1 hour. A HRP-conjugated Goat polyclonal to mouse IgG was used as secondary antibody at 1/1000 dilution.

  See Abreview