Anti-Mitotic Cells antibody [8B3G]

• 产品名称

  Anti-Mitotic Cells抗体[8B3G]

• 描述

  小鼠单克隆抗体[8B3G] to Mitotic Cells

• 宿主

  Mouse

• 特异性

  Strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) this antibody clearly distinguishes these M-phase cells from cell at other stages of the cell cycle. Dynamic information can be obtained by combining BrdU incorporation with antibody staining, which can distinguish and quantitate the four major fractions of the cell cycle. This antibody can be used for flow cytometric analyses and immunocytochemistry, it is not suitable for immunoblotting.

• 经测试应用

  适用于: Flow Cyt, IHC-Frmore details

• 种属反应性

  与反应: Human, Zebrafish
  

• 免疫原

  Tissue, cells or virus corresponding to Human Mitotic Cells. Total cell lysate of the human bladder carcinoma cell line T24.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  单克隆

• 克隆编号

  8B3G

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgM

The Abpromise guarantee

Abpromise™承诺保证使用ab8956于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• Flow Cytometry - Anti-Mitotic Cells antibody [8B3G] (ab8956)Dual parameter flow cytometric analysis of human colon cancer HT29 cells stained with monoclonal antibody 8B3G and propidium iodide (PI). The mitotic cell fraction is encircled.

   

• Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956)ab8956 staining a membrane-associated compound in proliferating (all) cells in tissue sections of 9 day old Zebrafish embryos by Immunohistochemistry (Frozen sections). Samples ware frozen sections fixed in Acetone:Methanol 1:1. ab8956 used at 1:50 dilution and incubated for 45 minutes at room temperature.

• Immunohistochemistry (Frozen sections) - Anti-Mitotic Cells antibody [8B3G] (ab8956)ab8956 staining a membrane-associated compound in proliferating (all) cells in tissue sections of 9 day old Zebrafish embryos by Immunohistochemistry (Frozen sections). Samples ware frozen sections fixed in Acetone:Methanol 1:1. ab8956 used at 1:50 dilution and incubated for 45 minutes at room temperature.