Anti-Notch2抗体
参阅全部 Notch2 一抗
兔多克隆抗体to Notch2
Rabbit
The Notch2 protein has many isoforms. The immunogenic sequence for the anti-Notch2 antibody is identical to the same region in full length (260+ kDa) as well as the cleaved forms ~82 kDa each (Notch 2 extracellular truncation & Notch 2 intracellular domain) and the post translationally modified forms thereof.
适用于: IHC-P, WBmore details
与反应: Human
预测可用于: Mouse, Rat
Synthetic peptide corresponding to Human Notch2 aa 1700-1800 (N terminal) (Cysteine residue). The epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
Database link: Q04721
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride, 98.6% Whole serum
多克隆
IgG
Abpromise™承诺保证使用ab8926于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/500. | |
| WB | 1/400 - 1/2000. Predicted molecular weight: 265 kDa. |
Entrez Gene: 4853 Human
Entrez Gene: 18129 Mouse
Omim: 600275 Human
SwissProt: Q04721 Human
SwissProt: O35516 Mouse
Unigene: 487360 Human
Unigene: 485843 Mouse
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (ab8926)Image from Murta, Daniel et al., PLoS ONE 8.8 (2013): e72767. Fig 2J. doi: 10.1371/journal.pone.0072767 P. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of CD-1 mice testes tissue sections labeling Notch2 with ab8926 at 1/100 dilution. CD-1 mice testes were fixed in 4% neutral phosphate buffered formalin at room temperature for 24 h and, after subsequent dehydration in ethanol, were embedded in paraffin. Tissue sections were stained by haematoxylin and identification of cell types was done through histology; The antigen retrieval step was performed in citrate buffer (10 mM, pH 6.0), Blocking was performed in PBS with 2% w/v bovine serum albumin for one hour at room temperature. Tissue sections were incubated overnight at 4°C with anti-Notch2 (ab8926). Positive immunostaining in brown color. Notch2 is expressed in germ cells entering meiosis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (ab8926)Image from Murta, Daniel et al., PLoS ONE 8.8 (2013): e72767. Fig 2J. doi: 10.1371/journal.pone.0072767 P.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of CD-1 mice testes tissue sections labeling Notch2 with ab8926 at 1/100 dilution. CD-1 mice testes were fixed in 4% neutral phosphate buffered formalin at room temperature for 24 h and, after subsequent dehydration in ethanol, were embedded in paraffin. Tissue sections were stained by haematoxylin and identification of cell types was done through histology; The antigen retrieval step was performed in citrate buffer (10 mM, pH 6.0), Blocking was performed in PBS with 2% w/v bovine serum albumin for one hour at room temperature. Tissue sections were incubated overnight at 4°C with anti-Notch2 (ab8926). Positive immunostaining in brown color. Notch2 is ubiquitously expressed in germ cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Notch2 antibody (ab8926)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Notch2 with ab8926 at 1/500 dilution. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
