Anti-Angiopoietin 1抗体
参阅全部 Angiopoietin 1 一抗
兔多克隆抗体to Angiopoietin 1
Rabbit
Anti-Angiopoietin 1 is directed against mouse angiopoietin-1 and shows no reactivity with mouse angiopoietin-2. This reagent cross-reacts with human angiopoietin 1. Partially cross reactivity is noted with human angiopoietin 2.
适用于: ICC/IF, IHC-P, WB, ELISAmore details
与反应: Mouse, Human, Pig
Synthetic peptide corresponding to Mouse Angiopoietin 1 aa 21-40 (N terminal).
Sequence:
CNQRRNPENGGRRYNRIQHGQ
Database link: O08538
Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver. To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody (and the ang-2 antibody, ab8452) give a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride, 98.6% Whole serum
his product was prepared from monospecific antiserum by a delipidation and defibrination.
Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver. To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody (and the ang-2 antibody, ab8452) give a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.
多克隆
IgG
Abpromise™承诺保证使用ab8451于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | (1) | 1/200 - 1/800. |
| WB | (1) | 1/500 - 1/2000. Detects a band of approximately 57 kDa. Using this antiserum with cell supernatants may result in high background due to other components in the serum. This can be alleviated by immunoprecipitating the antibody:antigen complex before detection, or precipitation using soluble Tie2. These methods result in a clean, strong signal. Both Ang-1 and Ang-2 proteins have predicted MW of 57 kDa and appear on blots close to predicted MW. Additional bands may be seen at approx. 75 kDa which represent highly glycosylated forms of the protein. Read More |
| ELISA | 1/5000 - 1/25000. |
Entrez Gene: 284 Human
Entrez Gene: 11600 Mouse
Omim: 601667 Human
SwissProt: Q15389 Human
SwissProt: O08538 Mouse
Unigene: 369675 Human
Unigene: 309336 Mouse
AGP 1 antibody
AGP1 antibody
AGPT antibody
ANG 1 antibody
ANG antibody
ANG-1 antibody
ANG1 antibody
Angiopoietin-1 antibody
Angiopoietin1 antibody
ANGP1_HUMAN antibody
ANGPT 1 antibody
Angpt1 antibody
KIAA0003 antibody
OTTHUMP00000227592 antibody
OTTHUMP00000227599 antibody
OTTHUMP00000227600 antibody
Western blot - Anti-Angiopoietin 1 antibody (ab8451)This image is courtesy of Marion Scharpfenecker 2002
Supernatants of mouse-angiopoietin-expressing endothelial cells.
Lane 1 - wt endothelial cell
Lane 2 - mouse Ang-1 (clone 1-8) expressing cells
Lane 3 - mouse Ang-1 (clone 1-15) expressing cells
Lane 4 - mouse Ang-2 (clone 2-9) expressing cells
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiopoietin 1 antibody (ab8451)
ab8451 was diluted 1:500 to detect Angiopoietin 1 in human lung tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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