Anti-Osteopontin antibody

• 产品名称

  Anti-Osteopontin抗体
  参阅全部 Osteopontin 一抗

• 描述

  兔多克隆抗体to Osteopontin

• 宿主

  Rabbit

• 特异性

  Osteopontin is directed against human osteopontin. The antibody recognizes the full-length osteopontin protein (which runs at 66 kDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 kDa MMP-cleaved C-fragment is recognized, but not the 40 kDa N-terminal fragment. Reactivity is reported to occur with osteopontin from swine, dog, mouse and rat.

• 经测试应用

  适用于: IHC-Pmore details
  不适用于: ICC/IF or WB

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide corresponding to Human Osteopontin aa 170-183 conjugated to keyhole limpet haemocyanin.
  Sequence:

  CKSKKFRRPDIQYPD

  
  Database link: P10451
  (Peptide available as ab9078)

  Run BLAST with Run BLAST with 

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride, 98.6% Whole serum

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab8448于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for ICC/IF or WB.

• 数据库链接

• Entrez Gene: 6696 Human

• Omim: 166490 Human

• SwissProt: P10451 Human

• Unigene: 313 Human

• 别名

• BSP I antibody

• BSPI antibody

• Early T lymphocyte activation 1 antibody

• ETA 1 antibody

• ETA1 antibody

• MGC110940 antibody

• Nephropontin antibody

• OPN antibody

• Osteopontin antibody

• osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody

• OSTP_HUMAN antibody

• PSEC0156 antibody

• secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1) antibody

• secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody

• Secreted phosphoprotein 1 antibody

• SPP 1 antibody

• SPP-1 antibody

• SPP1 antibody

• SPP1/CALPHA1 fusion antibody

• Urinary stone protein antibody

• Uropontin antibody

• BNSP antibody

• Bone sialoprotein 1 antibody

• Bone sialoprotein I antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (ab8448)

  Human Breast tumour section. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section). There is also weak staining of the extracellular matrix. Osteopontin is not believed to be expressed inside breast tumour cells, and there is no staining in the intracellular region of the breast cells in this section.

  Osteopontin antibody (ab8448) used at 1:100-1:300. No antigen retrieval is required.

• 

  Schematic Diagram - Anti-Osteopontin antibody (ab8448)This image is courtesy of Agnihotri et al, JBC 2001

  OPN is cleaved by MMP to yield 2 fragments, which migrate at 40kD(N terminal) and 32kD (C terminal). The C terminal fragment can undergo further cleavage by both of these MMPs (see Agnihotri et al, JBC 2001 for further details). The epitope recognised by ab8448 is shown in violet. This antibody detects the full length OPN and the 32kD fragment. It does not recognise the 40kD fragment.