Anti-DRAK2 antibody

• 产品名称

  Anti-DRAK2抗体
  参阅全部 DRAK2 一抗

• 描述

  兔多克隆抗体to DRAK2

• 宿主

  Rabbit

• 经测试应用

  适用于: WB, ICC/IFmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide:

  CSKRFRFDDSLPNPHE

  , corresponding to amino acids 351/365 of Human DRAK2. (Peptide available as ab8454.)

  Run BLAST with Run BLAST with 

• 阳性对照

• Wholecell lysate from Jurkat cells An approximately 45 kDa band can be detected.

• 常规说明

  
  

  Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP,are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases) (1). DRAKs contain anN-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

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• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C.

• 存储溶液

  pH: 7.2
  Preservative: 0.02% Sodium azide

• 纯度

  Ion Exchange Chromatography

• 纯化说明

  DRAK2 Antibody is Ion exchange chromatography purified.

• Primary antibody说明

  Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP,are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases) (1). DRAKs contain anN-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues.

• 克隆

  多克隆

• 同种型

  IgG

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab8419于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 9262 Human

• Omim: 604727 Human

• SwissProt: O94768 Human

• Unigene: 88297 Human

• 别名

• Death associated protein kinase related 2 antibody

• DRAK 2 antibody

• DRAK2 antibody

• Serine/threonine kinase 17b antibody

• Serine/threonine kinase 17b apoptosis inducing antibody

• Serine/threonine protein kinase 17B antibody

• Serine/threonine-protein kinase 17B antibody

• ST17B_HUMAN antibody

• STK 17B antibody

• Stk17b antibody

• DAP kinase related apoptosis inducing protein antibody

• DAP kinase related apoptosis inducing protein kinase 2 antibody

• DAP kinase-related apoptosis-inducing protein kinase 2 antibody

• 

  Western blot - Anti-DRAK2 antibody (ab8419)

  Lane 1 : Anti-DRAK2 antibody (ab8419) at 1 µg/ml (DRAK2 antibody)
  Lane 2 : Anti-DRAK2 antibody (ab8419) at 2 µg/ml (DRAK2 antibody)
  
  All lanes : Raji cell lysate
  
  Predicted band size: 43 kDa
  
  

• 

  Western blot - Anti-DRAK2 antibody (ab8419)

  All lanes : Anti-DRAK2 antibody (ab8419) at 1/500 dilution
  
  Lane 1 : Jurkat whole cell lysate with absence of blocking peptide
  Lane 2 : Raji whole cell lysate with absence of blocking peptide
  Lane 3 : Jurkat whole cell lysate with presence of blocking peptide
  Lane 4 : Raji whole cell lysate with presence of blocking peptide
  
  Predicted band size: 43 kDa
  Observed band size: 45 kDawhy is the actual band size different from the predicted?
  
  
  
  We are unsure about the nature of the 70 kDa band. However, DRAK2 is autophosphorylated and it is possible that this band corresponds to the phosphorylated form. The fact that the detection of this band is blocked by DRAK2 peptide indicates that it probably is closely related to the DRAK2 protein.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-DRAK2 antibody (ab8419)

  ab8419 at 10µg/ml staining DRAK2 in Jurkat cells by ICC/IF