Anti-DR5抗体
参阅全部 DR5 一抗
兔多克隆抗体to DR5
Rabbit
Does not cross react with DR4.
适用于: IHC-P, WB, ICCmore details
与反应: Mouse, Rat, Human
Synthetic peptide corresponding to Human DR5 (C terminal). DR5 antibody was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of human DR5 precursor. The immunogen is located within the last 50 amino acids of DR5.
Database link: O14763
(Peptide available as ab8449)
WB: HEK293, A431, A549, Caco-2, Daudi, HeLa, HepG2, K562, MCF7, Jurkat, SK-N-SH and THP-1, 3T3/NIH, C2-C12 cell lysates (Human, Mouse, Rat). IHC-P: Mouse kidney tissue and rat brain tissue. ICC: HeLa cells.
For information on sample preparation for western blot, please refer here.
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Liquid
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at +4°C.
pH: 7.2
Preservative: 0.02% Sodium azide
浓度
100 µg 浓度为 1 mg/ml
Affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab8416于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) | Use a concentration of 5 µg/ml. |
| WB | (3) | Use a concentration of 2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 47.8 kDa). |
| ICC | Use a concentration of 5 µg/ml. |
Entrez Gene: 8795 Human
Entrez Gene: 21933 Mouse
Omim: 603612 Human
SwissProt: O14763 Human
SwissProt: Q9QZM4 Mouse
Unigene: 521456 Human
Unigene: 661668 Human
Unigene: 193430 Mouse
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Western blot - Anti-DR5 antibody (ab8416)
All lanes : Anti-DR5 antibody (ab8416) at 0.5 µg/ml
Lane 1 : HEK293 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Caco-2 cell lysate
Lane 5 : Daudi cell lysate
Lane 6 : HeLa cell lysate
Lane 7 : HepG2 cell lysate
Lane 8 : K562 cell lysate
Lane 9 : MCF7 cell lysate
Lane 10 : Jurkat cell lysate
Lane 11 : SK-N-SH cell lysate
Lane 12 : THP-1 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 47.8 kDa
Western blot - Anti-DR5 antibody (ab8416)
All lanes : Anti-DR5 antibody (ab8416) at 1 µg
Lane 1 : 3T3/NIH cell lysate
Lane 2 : C2C12 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 47.8 kDa
Western blot - Anti-DR5 antibody (ab8416)
Anti-DR5 antibody (ab8416) at 1 µg/ml + Rat Skeletal Muscle at 15 µg
Secondary
Goat Anti-Rabbit IgG HRP Conjugate at 1/10000 dilution
Predicted band size: 47.8 kDa
Blocking buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody (ab8416)
Immunohistochemical analysis of 4% paraformaldehydefixed mouse kidney tissue using anti-DR5 antibody (ab8416) at 20µg/mL followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody (ab8416)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-DR5 antibody (ab8416) at 5µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation (microwave for 20 minutes) with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary at room temperature for 1 hour. Counter stained with Hematoxylin.
Endogenous peroxidase blocking: 3% H202 at room temperature for 10 minutes.
Washing: PBS, 2 x 5 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DR5 antibody (ab8416)
IHC-P of 4% paraformaldehyde-fixed rat brain tissue labelled DR5 with ab8416 at 5µg/ml, followed by Goat Anti-Rabbit IgG secondary antibody at 1/5000 dilution (green) and DAPI (blue).
Immunocytochemistry - Anti-DR5 antibody (ab8416)
ICC analysis of 4% paraformaldehyde-fixed (room temperature for 10 minutes) HeLa cells labeling DR5 with ab8416 at 20µg/mL, followed by goat anti-rabbit IgG antibody (Alexa Fluor 488) at 1/500 dilution (green).
Blocking condition: 5% BSA, room temperature for 1 hour.
Primary antibody incubation: 4°C overnight.
Secondary antibody incubation: room temperature for 1 hour.
Washing condition: PBS, 3 x 3 minutes
Immunocytochemistry - Anti-DR5 antibody (ab8416)
ab8416 at 5µg/ml staining DR5 in Hela cells by ICC. Cells were fixed with formaldehyde and blocked with 10% serum for 1 hour at room termperature. Antigen retrieval was by heat mediation with a Citrate buffer (pH 6). Overnight primary antibody at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary.
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