Anti-Salmonella typhimurium LPS抗体[1E6]
小鼠单克隆抗体[1E6] to Salmonella typhimurium LPS
Mouse
This antibody is directed against lipopolisaccharides of Salmonella typhimurium
适用于: WB, Flow Cyt, Dot blot, ELISA, ICC/IF, Electron Microscopymore details
与反应: Salmonella typhimurium
Full length native protein (purified) corresponding to Salmonella typhimurium Salmonella typhimurium LPS. Lipopolysaccharides of Salmonella typhimurium
This antibody is used for the detection of LPS of Salmonella typhimurium.
This product was changed from ascites to tissue culture supernatant on 16th July 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.40
Preservative: 0.1% Sodium azide
Protein A purified
This antibody is used for the detection of LPS of Salmonella typhimurium
单克隆
1E6
Sp2/0
IgG1
unknown
Abpromise™承诺保证使用ab8274于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | Use at an assay dependent concentration. |
| Flow Cyt | Use at an assay dependent concentration. PubMed: 17442963 ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. | |
| Dot blot | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| Electron Microscopy | Use at an assay dependent concentration. |
S. typhimurium antibody
Salmonella typhimurium antibody
SALMONELLA TYPHIMURIUM LPS FACTOR 4 antibody
![Electron Microscopy - Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274)](https://www.abcam.cn/ps/products/8/ab8274/Images/ab8274-14006-anti-salmonella-typhimurium-lps-antibody-1e6-immunohistochemistry.jpg)
Electron Microscopy - Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274)This image is courtesy of an anonymous Abreview
Electron microscopy image of ultrathin cryosections of Salmonella paratyphi using ab8274 (1/100). For further experimental details please refer to abreview.
![Western blot - Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274)](https://www.abcam.cn/ps/products/8/ab8274/Images/ab8274-14005-anti-salmonella-typhimurium-lps-antibody-1e6-western-blot.jpg)
Western blot - Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274)Image courtesy of Aaron White by Abreview.
All lanes : Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274) at 1/1000 dilution
Lane 1 : Whole cell polysaccharides from S. Enteritidis 27655-3b
Lane 2 : Whole cell polysaccharides from S. Enteritidis Sal8
Lane 3 : Whole cell polysaccharides from S. Typhimurium ATCC 14028s
Lane 4 : Whole cell polysaccharides from S. Typhimurium SL1344
Lysates/proteins at 100000000 cells per lane.
Secondary
All lanes : AP conjugated goat anti-mouse polyclonal at 1/4000 dilution
Gels were (A) silver-stained or (B) Western blotted using the Anti-Salmonella typhimurium 0-4 antibody [1E6] (ab8274) as the primary antibody. Although equal amounts of cellular material were detected by silver staining, only the S. Typhimurium samples were recognized by the 1E6 antibody on the corresponding Western blot.
