Anti-CD18 antibody [MEM-148]

• 产品名称

  Anti-CD18抗体[MEM-148]
  参阅全部 CD18 一抗

• 描述

  小鼠单克隆抗体[MEM-148] to CD18

• 宿主

  Mouse

• 特异性

  Recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1.

• 经测试应用

  适用于: WB, Flow Cytmore details

• 种属反应性

  与反应: Human
  不与反应: Pig, Monkey

• 免疫原

  Tissue, cells or virus corresponding to Human CD18. Peripheral blood mononuclear cells

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  MEM-148

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab8220于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3689 Human

• Omim: 600065 Human

• SwissProt: P05107 Human

• Unigene: 375957 Human

• 别名

• CD18 antibody

• Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody

• Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody

• Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody

• Complement component 3 receptor 3 and 4 subunit antibody

• Complement receptor C3 beta subunit antibody

• Complement receptor C3 subunit beta antibody

• Integrin beta 2 antibody

• Integrin beta chain beta 2 antibody

• Integrin beta-2 (ITGB2) antibody

• Integrin beta-2 antibody

• Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody

• ITB2_HUMAN antibody

• ITGB2 antibody

• LAD antibody

• LCAMB antibody

• Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody

• Leukocyte cell adhesion molecule CD18 antibody

• LFA 1 antibody

• LFA1 antibody

• Lymphocyte function associated antigen 1 (LFA1) antibody

• Lymphocyte function associated antigen 1 antibody

• MAC 1 antibody

• MAC1 antibody

• Macrophage antigen 1 (mac1) beta antibody

• MF17 antibody

• MFI7 antibody

• OTTHUMP00000115278 antibody

• OTTHUMP00000115279 antibody

• OTTHUMP00000115280 antibody

• OTTHUMP00000115281 antibody

• OTTHUMP00000115282 antibody

• 95 subunit beta antibody

• Beta 2 (antigen CD18 (p95) antibody

• CD 18 antibody

• 

  Flow Cytometry - Anti-CD18 antibody [MEM-148] (ab8220)

  Flow cytometry analysis of human peripheral whole blood cells using ab8220 at 1.67 µg/ml showing separation of human monocytes (red-filled) from CD18 negative blood debris (black-dashed).

• 

  Flow Cytometry - Anti-CD18 antibody [MEM-148] (ab8220)

  Human peripheral blood lymphocytes stained with ab8220 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab8220, 0.1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.