Anti-Neurofilament heavy polypeptide antibody

• 产品名称

  Anti-Neurofilament heavy polypeptide抗体
  参阅全部 Neurofilament heavy polypeptide 一抗

• 描述

  兔多克隆抗体to Neurofilament heavy polypeptide

• 宿主

  Rabbit

• 特异性

  The antibody recognizes both phosphorylated and non-phosphorylated forms of NF-H. Specifically recognizes the heavy microfilament subunit (~180-220 kDa).

• 经测试应用

  适用于: IHC-FrFl, WBmore details

• 种属反应性

  与反应: Mouse, Rat
  

• 免疫原

  Full length native protein (purified) corresponding to Cow Neurofilament heavy polypeptide. From bovine spinal cord.
  Database link: F1MSQ6

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.03% Sodium azide

• 纯度

  Whole antiserum

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab8135于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 380684 Mouse

• Entrez Gene: 24587 Rat

• SwissProt: P19246 Mouse

• SwissProt: P16884 Rat

• Unigene: 298283 Mouse

• Unigene: 108194 Rat

• 别名

• Neurofilament heavy polypeptide 200kDa antibody

• Neurofilament heavy polypeptide antibody

• Neurofilament triplet H protein antibody

• NF H antibody

• NF-H antibody

• NFH antibody

• NFH_HUMAN antibody

• 200 kDa neurofilament protein antibody

• CMT2CC antibody

• Nefh antibody

• 

  Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (ab8135)

  Immunohistological analysis of a mouse hippocampus section stained with ab8135 at a dilution 1:2,000 in red, and co-stained with a mouse mAb to myelin basic protein (MBP) at a dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA.

  Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 μM, and free-floating sections were stained.

• 

  Western blot - Anti-Neurofilament heavy polypeptide antibody (ab8135)

  All lanes : Anti-Neurofilament heavy polypeptide antibody (ab8135) at 1/10000 dilution
  
  Lane 2 : Rat brain tissue lysate
  Lane 3 : Rat spinal cord tissue lysate
  Lane 4 : Mouse brain tissue lysate
  Lane 5 : Mouse spinal cord tissue lysate