Anti-Synaptophysin antibody [SY38]

• 产品名称

  Anti-Synaptophysin抗体[SY38]
  参阅全部 Synaptophysin 一抗

• 描述

  小鼠单克隆抗体[SY38] to Synaptophysin

• 宿主

  Mouse

• 经测试应用

  适用于: WB, ICC/IF, IHC-Fr, Flow Cyt, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Rat, Hamster, Cow, Human
  

• 免疫原

  Full length native protein (purified) corresponding to Bovine Synaptophysin. Synaptophysin from presynaptic vesicles, prepared from bovine brain.
  Database link: P20488

• 阳性对照

• Adrenal pheochromocytoma and pancreatic islet cell neoplasm

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C.

• 存储溶液

  pH: 7.3
  Preservative: 0.09% Sodium azide
  Constituents: 0.5% BSA, PBS

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  SY38

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab8049于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 280937 Cow

• Entrez Gene: 6855 Human

• Entrez Gene: 20977 Mouse

• Entrez Gene: 24804 Rat

• Omim: 313475 Human

• SwissProt: P20488 Cow

• SwissProt: P08247 Human

• SwissProt: Q62277 Mouse

• SwissProt: P07825 Rat

• Unigene: 632804 Human

• Unigene: 223674 Mouse

• Unigene: 11067 Rat

别名

• Major synaptic vesicle protein p38 antibody

• MRX96 antibody

• MRXSYP antibody

• Syn p38 antibody

• Synaptophysin antibody

• Syp antibody

• SYPH antibody

• SYPH_HUMAN antibody

• SypI antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)This image is courtesy of an Abreview provided by Carl Hobbs

  ab8049 staining Synaptophysin in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/100) for 16 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

  See Abreview

• 

  Western blot - Anti-Synaptophysin antibody [SY38] (ab8049)

  All lanes : Anti-Synaptophysin antibody [SY38] (ab8049) at 1/500 dilution
  
  Lane 1 : Human brain tissue lysate - total protein (ab29466)
  Lane 2 : Brain (Mouse) Tissue Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed at 1/3000 dilution
  
  Predicted band size: 34 kDa
  Observed band size: 38 kDawhy is the actual band size different from the predicted?
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)This image is courtesy of an Abreview provided by Carl Hobbs.

  ab8049 staining MAFA in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/100 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouseIgG polyclonal (1/300) was used as the secondary antibody.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)

  The pictures show immunohistochemical staining of normal mucosa and neuroendocrine tumour of the small intestine.  The antibody (ab8049) stains positive in a 1:10 dilution in nerve cells of the colon as well as some cells in normal colon mucosa and some neuroendocrine tumors of the digestive tract. Staining protocol: Mouse monoclonal to Synaptophysin (ab8049), diluted 1:10 in PBS plus BSA, 60 min at room temperature,  
  secondary antibody: HRP solution with anti mouse polyclonal antibodies, 30min. DAB staining 10 min; Hematoxylin 30 sec.

  These images were kindly supplied as part of the review submitted by Karin Birkenkamp-Demtroeder.

• 

  Flow Cytometry - Anti-Synaptophysin antibody [SY38] (ab8049)

  Overlay histogram showing PC12 cells stained with ab8049 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8049, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.