Anti-Jagged1抗体
参阅全部 Jagged1 一抗
兔多克隆抗体to Jagged1
Rabbit
A BLAST analysis was used to suggest cross reactivity with Jagged-1 protein from human, chimpanzee, rat and mouse based on 100% homology with the immunizing sequence. Partial reactivity is expected against dog (81%) and Xenopus laevis (85%) based on partial sequence homologies as indicated. Reactivity against homologues from other sources is not known.
适用于: ICC/IF, IHC-P, WBmore details
与反应: Mouse, Human
预测可用于: Rat
Synthetic peptide: N-KASRGNDRNRIVLPFS conjugated to KLH, corresponding to amino acids 110-125 of Human Jagged1
WB: Mouse liver lysate. IHC: Human cervical and human cervical cancer tissues. ICC/IF: Human corneal epithelial cells
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
Protein A purified
This protein A purified antibody is directed against human Jagged-1 protein. The product was purified from mono-specific antiserum by affinity chromatography.
多克隆
IgG
Abpromise™承诺保证使用ab7771于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) | 1/200 - 1/1000. |
| IHC-P | (4) | 1/100 - 1/500. |
| WB | (2) | 1/500 - 1/2000. Detects a band of approximately 150 kDa (predicted molecular weight: 134 kDa). 150 kDa in mouse liver lysate |
Entrez Gene: 182 Human
Entrez Gene: 16449 Mouse
Omim: 601920 Human
SwissProt: P78504 Human
SwissProt: Q9QXX0 Mouse
Unigene: 224012 Human
Unigene: 22398 Mouse
Unigene: 88804 Rat
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (ab7771)This image is courtesy of the Martin Kast Laboratory
Formalin-fixed paraffin embedded IHC of normal human cervial tissues (40x magnification) stained with ab7771 (1/100 dilution). The slides were counterstained with hematoxylin
Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody (ab7771)This image is courtesy of Aihua Ma, Univdersity of Cardiff
Staining of Human corneal epithelial cells with anti-Jagged1 (ab7771, 1/500). The Jagged1 (green staining) is localised to the cytoplasm and cell nucleus and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (1/500, blue staining).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (ab7771)This image is courtesy of the Martin Kast Laboratory
Formalin-fixed paraffin embedded IHC of human cervical cancer tissues (40x magnification) stained with ab7771 (1/100 dilution). The slides were counterstained with hematoxylin
Western blot - Anti-Jagged1 antibody (ab7771)
ab7771 Rabbit polyclonal to Jagged1 (1/500) with secondary Goat anti-rabbit IgG antibody ab6721 (1/5000)
Exposure time: 1 minute. Expected molecular weight: 134 kDa
Lanes 1 to 3 and 5: 20µg of cell lysate per lane
Lane 4: 20 µl of 1x LDS loading buffer
Lane 1: Human Brain tissue lysate
Lane 2: Human Kidney tissue Lysate
Lane 3: Human liver tissue lysate
Lane 4: 1x LDS loading buffer
Lane 5: Mouse liver lysate
