Anti-HLA G抗体[MEM-G/1]
参阅全部 HLA G 一抗
小鼠单克隆抗体[MEM-G/1] to HLA G
Mouse
The antibody reacts with denaturated HLA-G heavy chain.
适用于: IHC-P, WBmore details
与反应: Human
Recombinant full length denatured heavy chain (Human).
IHC-P: First trimester placenta and pulmonary diseases. WB: ab29745; HeLa and JEG-3 cell lysates.
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS
Protein A purified
Purified from TCS. Purity >95% by SDS-PAGE.
单克隆
MEM-G/1
IgG1
Abpromise™承诺保证使用ab7759于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) | 1/60 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
| WB | (1) | Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 38.2 kDa. |
Entrez Gene: 3135 Human
Omim: 142871 Human
SwissProt: P17693 Human
Unigene: 512152 Human
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)](https://www.abcam.cn/ps/products/7/ab7759/Images/ab7759-454758-anti-hla-g-antibody-mem-g1-immunohistochemistry-placenta-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)
Immunohistochemistry staining with ab7759 of first trimester placenta (paraffin-embedded sections).
![Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)](https://www.abcam.cn/ps/products/7/ab7759/Images/ab7759-13067-anti-hla-g-antibody-mem-g-1-western-blot.jpg)
Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)
All lanes : Anti-HLA G antibody [MEM-G/1] (ab7759) at 10 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 38.2 kDa
Observed band size: 42 kDawhy is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)](https://www.abcam.cn/ps/products/7/ab7759/Images/ab7759_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA G antibody [MEM-G/1] (ab7759)
The antibody ab7759 stains infiltrating macrophages in pulmonary diseases. In the top left corner see detail of macropage.
![Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)](https://www.abcam.cn/ps/products/7/ab7759/Images/ab7759-481938-antihla-g-antibody-memg-1-western-blot-jeg3.jpg)
Western blot - Anti-HLA G antibody [MEM-G/1] (ab7759)
All lanes : Anti-HLA G antibody [MEM-G/1] (ab7759) at 2 µg/ml
Lane 1 : JEG-3 cell line under reducing conditions.
Lane 2 : LNCap cell line under reducing conditions.
Lane 3 : JEG-3 cell line under non-reducing conditions.
Lane 4 : LNCap cell line under non-reducing conditions.
Secondary
All lanes : IRDye800-conjugated anti-mouse secondary antibody
Predicted band size: 38.2 kDa
Observed band size: 40 kDawhy is the actual band size different from the predicted?
Western blotting analysis of human HLA-G using ab7759 on lysates of JEG-3 cell line and LNCaP cell line (negative control) under reducing and non-reducing conditions.
Nitrocellulose membrane was probed with 2 µg/ml of ab7759 followed by IRDye800-conjugated anti-mouse secondary antibody.
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