Anti-alpha 1 Sodium Potassium ATPase antibody [464.6]

• 产品名称

  Anti-alpha 1 Sodium Potassium ATPase抗体[464.6]
  参阅全部 alpha 1 Sodium Potassium ATPase 一抗

• 描述

  小鼠单克隆抗体[464.6] to alpha 1 Sodium Potassium ATPase

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, IHC-P, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Rabbit, Human, Pig
  预测可用于: Sheep, Dog, Xenopus laevis, Monkey

• 免疫原

  Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• This antibody gave a positive signal in the following tissue lysates: human kidney, rabbit heart, human brain and human brain membrane.This antibody also gave a positive signal in Porcine proximal tubule lysate.

• 常规说明

  This antibody clone [464.6] is manufactured by Abcam.

  If you require this antibody in a different buffer formulation or a different conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine
  
  Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team.

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  464.6

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab7671于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• SwissProt: P05023 Human

• SwissProt: Q8VDN2 Mouse

• SwissProt: P06685 Rat

• SwissProt: Q92123 Xenopus laevis

• Unigene: 371889 Human

• Unigene: 280103 Mouse

• Unigene: 217534 Rat

• Unigene: 2992 Rat

• Unigene: 3792 Xenopus laevis

• Entrez Gene: 403992 Dog

• Entrez Gene: 476 Human

• Entrez Gene: 11928 Mouse

• Entrez Gene: 397481 Pig

• Entrez Gene: 24211 Rat

• Entrez Gene: 443381 Sheep

• Entrez Gene: 399285 Xenopus laevis

• Omim: 182310 Human

• 别名

• ATP1A1 antibody

• Atpa-1 antibody

• ATPase Na+/K+ transporting alpha 1 polypeptide antibody

• ATPase Na+/K+ transporting subunit alpha 1 antibody

• BC010319 antibody

• EC 3.6.3.9 antibody

• MGC3285 antibody

• MGC38419 antibody

• MGC51750 antibody

• Na K ATPase alpha A catalytic polypeptide antibody

• Na K ATPase catalytic subunit alpha A protein antibody

• Na(+)/K(+) ATPase 1 antibody

• Na(+)/K(+) ATPase alpha-1 subunit antibody

• Na+, K+ ATPase alpha subunit antibody

• Na+/K+ ATPase alpha 1 subunit antibody

• Na+/K+ ATPase 1 antibody

• Na,K ATPase alpha 1 subunit antibody

• Nkaa1b antibody

• Sodium potassium ATPase alpha 1 polypeptide antibody

• Sodium pump 1 antibody

• Sodium pump subunit alpha-1 antibody

• sodium-potassium ATPase catalytic subunit alpha-1 antibody

• Sodium/potassium-transporting ATPase subunit alpha-1 antibody

• A1A1 antibody

• AT1A1 antibody

• AT1A1_HUMAN antibody

• 

  Immunocytochemistry - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)This image is courtesy of an Abreview submitted by Armen Petrosyan

  Immunocytochemistry analysis of formaldehyde-fixed mouse hepatocytes permeabilized with 0.2% Triton X-100 in PBS staining with ab7671 at 1/50 dilution. Secondary antibody was Alexa Fluor™ 488 Donkey anti-Ms. Samples were incubated with the primary antibody with 1% donkey serum in PBST for 3 hours at 22°C. Blocking was done using 1% donkey serum in PBST for 1 hour at 22°C.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)

  ab7671 staining alpha 1 Sodium Potassium ATPase in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab7671 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  Also suitable in cells fixed with 100% methanol (5 min).

  Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a single confocal section is shown.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)This image is courtesy of an Abreview submitted by Anne Sailer

  ab7671 staining alpha 1 Sodium Potassium ATPase in human formaldehyde tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 12 hours at 4°C; antigen retrieval was by heat mediation in a buffer pH 9. Samples were incubated with primary antibody (5µg/ml in 5% BSA) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

  See Abreview

• 

  Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)

  Lanes 1-3 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1 µg/ml
  Lanes 4-6 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 2 µg/ml
  Lanes 7-9 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 5 µg/ml
  
  Lanes 1 & 4 & 7 : Human brain tissue lysate - total protein (ab29466)
  Lanes 2 & 5 & 8 : Brain (Mouse) Tissue Lysate (ab27253)
  Lanes 3 & 6 & 9 : Brain (Rat) Tissue Lysate (ab7942)
  
  Lysates/proteins at 20 µg per lane.
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 112 kDa
  
  
  Exposure time: 3 minutes
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)

  IHC image of Ab7671 staining in Human Kidney Carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab7671, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)

  All lanes : alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker at 10 µg/ml
  
  Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
  Lane 2 : Human kidney tissue lysate - total protein (ab30203)
  Lane 3 : Heart (Rabbit) Whole Cell Lysate - normal tissue (ab29072)
  Lane 4 : Human brain tissue lysate - total protein (ab29466)
  Lane 5 : Brain (Human) Membrane Lysate - adult normal tissue
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 112 kDa
  Observed band size: 100 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 3 minutes
  

• 

  Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671)

  Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1/5000 dilution + Porcine proximal tubule lysate
  
  Predicted band size: 112 kDa
  
  
  
  Western blot analysis detecting Na, K-ATP-ase (alpha) in porcine proximal tubule protein, using Ab7671. Band is at 112 kDa.