Anti-Slit2抗体
参阅全部 Slit2 一抗
兔多克隆抗体to Slit2
Rabbit
A BLAST analysis was used to suggest reactivity with this protein from human and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-2 from Tetraodon nigroviridis , as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). The core 13 amino acids of the immunogen are also 100% homologous to SLIT-2 from mouse, rat, bovine and Xenopus laevis. Cross reactivity with SLIT-2 homologues from other sources has not been determined.
适用于: WB, ICC/IF, IHC-Frmore details
与反应: Mouse, Rat, Chicken, Human
预测可用于: Xenopus laevis
Synthetic peptide:
YFIPGTEDYRSKLSGDC
conjugated to KLH, corresponding to amino acids 484-500 of Human SLIT2.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1 mg/ml
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
This affinity purified antibody is directed against human SLIT-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
多克隆
IgG
Abpromise™承诺保证使用ab7665于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/2000. Detects a band of approximately 165 kDa (predicted molecular weight: 170 kDa). Incubate primary antibody blocking buffer (5% goat serum + 0.5% non-fat dry milk in PBS) other blocking buffers cause a poor signal. | |
| ICC/IF | (4) | Use at an assay dependent concentration. |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 20498081 |
hide
Unigene: 29802 Human
Unigene: 289739 Mouse
Entrez Gene: 373967 Chicken
Entrez Gene: 9353 Human
Entrez Gene: 20563 Mouse
Entrez Gene: 394404 Xenopus laevis
Omim: 603746 Human
SwissProt: O94813 Human
SwissProt: Q9R1B9 Mouse
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OTTHUMP00000158695 antibody
OTTHUMP00000217852 antibody
OTTHUMP00000217853 antibody
OTTHUMP00000217854 antibody
Slil 3 antibody
Slil3 antibody
Slit 2 antibody
Slit homolog 2 (Drosophila) antibody
Slit homolog 2 antibody
Slit homolog 2 protein antibody
Slit homolog 2 protein C-product antibody
Slit-2 antibody
Slit2 antibody
SLIT2_HUMAN antibody
Drad 1 antibody
E030015M03Rik antibody
E130320P19Rik antibody
Western blot - Anti-Slit2 antibody (ab7665)
Anti-Slit2 antibody (ab7665) at 1/1350 dilution + Chicken spinal cord whole cell lysate at 30 µg
Secondary
IRDye™800
conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size: 170 kDa
Observed band size: 165 kDawhy is the actual band size different from the predicted?
Molecular weight estimation was made by comparison to prestained molecular weight markers
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