Anti-ABCA1抗体
参阅全部 ABCA1 一抗
兔多克隆抗体to ABCA1
Rabbit
ab7360 has previously been shown to work well in ICC/IF on HeLa cells. However recent batches of this antibody, do not work in this application. We would recommend ab18180 for researchers wanting to detect ABCA1 in ICC/IF. For further information, please contact our Scientific Support Team.
适用于: WB, IHC-Pmore details
与反应: Mouse, Human
Synthetic peptide corresponding to Human ABCA1. Synthetic peptide: The immunogen is generated from within residues 1100-1300 of Human ABCA1
Database link: O95477
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Liquid
Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
浓度
100 µl 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab7360于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (5) | 1/500 - 1/1000. Predicted molecular weight: 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABC1 signal. It is important not to boil the sample before loading onto the gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter into the gel. Read More |
| IHC-P | 1/500. |
Entrez Gene: 19 Human
Entrez Gene: 11303 Mouse
Omim: 600046 Human
SwissProt: O95477 Human
SwissProt: P41233 Mouse
Unigene: 429294 Human
Unigene: 277376 Mouse
ABC1 antibody
ABCA 1 antibody
ABCA1 antibody
ABCA1_HUMAN antibody
ATP binding Cassette 1 antibody
ATP binding cassette sub family A ABC1 member 1 antibody
ATP binding cassette sub family A member 1 antibody
ATP binding Cassette Transporter 1 antibody
ATP-binding cassette 1 antibody
ATP-binding cassette sub-family A member 1 antibody
ATP-binding cassette transporter 1 antibody
CERP antibody
Cholesterol efflux regulatory protein antibody
FLJ14958 antibody
HDLDT1 antibody
Membrane bound antibody
MGC164864 antibody
MGC165011 antibody
TD antibody
TGD antibody
ABC 1 antibody
ABC Transporter 1 antibody
ABC-1 antibody
Western blot - Anti-ABCA1 antibody (ab7360)
All lanes : Anti-ABCA1 antibody (ab7360)
Lane 1 : RAW 264.7 whole cell lysate treated with T0901317
Lane 2 : RAW 264.7 whole cell lysate untreated
Lysates/proteins at 25 µg per lane.
Predicted band size: 220 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody (ab7360)
ab7360, staining ABCA1 in human prostate epithelium showing luminal and membrane staining by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Western blot - Anti-ABCA1 antibody (ab7360)
The experiment was performed by treating RAW macrophages with 9-cis-retinoic acid and 22R-hydroxycholesterol, known inducers of ABCA1expression in macrophages. Then total cell post-nuclear lysate (40ug protein) was separated by SDS-PAGE and detected using a 1:1000 dilution of ab7360 affinity purified Lot G incubated for 1 hour at room temperature (Lane A). Although there are lower molecular weight bands on the blot, the ABCA1 signal is excellent and gives the expected 3 bands. It is not known why ABCA1 runs as three bands, but it has been found to do so by many researchers. It is probably due to protein modifications such as glycosylation. The antibody was also tested against ABCA1 transiently expressed in 293 cells as an independent test with excellent results.
The experiment was performed by treating RAW macrophages with 9-cis-retinoic acid and 22R-hydroxycholesterol, known inducers of ABCA1expression in macrophages. Then total cell post-nuclear lysate (40ug protein) was separ
Western blot - Anti-ABCA1 antibody (ab7360)
Detection of ABCA1 in mouse peritoneal macrophages using ab7360. ECL exposure, 1 min.
Lane 4: T09 uninduced lysate
Lane 5: T09 induced lysate Detection of ABCA1 in mouse peritoneal macrophages using ab7360. ECL exposure, 1 min.
Lane 4: T09 uninduced lysate
Lane 5: T09 induced lysate
