Anti-Myelin Basic Protein antibody [12]

• 产品名称

  Anti-Myelin Basic蛋白抗体[12]
  参阅全部 Myelin Basic Protein 一抗

• 描述

  大鼠单克隆抗体[12] to Myelin Basic蛋白

• 宿主

  Rat

• 经测试应用

  适用于: ICC/IF, WB, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Full length protein corresponding to Cow Myelin Basic Protein.

• 表位

  Amino acids 82-87 (DENPVV).

• 阳性对照

• WB: Human cerebellum, Mouse brain tissue and Rat brain tissue. IHC-P: Human cerebellum tissue. ICC/IF: Mouse primary oligodendroglia cells.

• 常规说明

  This product has switched from a hybridoma to recombinant production method on 08th November 2021.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

• 浓度

• 100 µg 浓度为 1.034 mg/ml

• 1 mg 浓度为 1.034 mg/ml

• 40 µg 浓度为 1.034 mg/ml

• 纯度

  Ion Exchange Chromatography

• 克隆

  单克隆

• 克隆编号

  12

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab7349于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 4155 Human

• Entrez Gene: 17196 Mouse

• Entrez Gene: 24547 Rat

• Omim: 159430 Human

• SwissProt: P02686 Human

• SwissProt: P04370 Mouse

• SwissProt: P02688 Rat

• Unigene: 551713 Human

• Unigene: 252063 Mouse

• Unigene: 392350 Mouse

• Unigene: 454459 Mouse

别名

• GDB antibody

• Golli MBP antibody

• Golli MBP; myelin basic protein antibody

• Hemopoietic MBP antibody

• HMBPR antibody

• HUGO antibody

• MBP antibody

• MBP_CAVPO antibody

• MBP_HUMAN antibody

• MGC99675 antibody

• MLD antibody

• Myelin A1 protein antibody

• Myelin A1 Protein, basic antibody

• Myelin basic protein antibody

• Myelin Deficient antibody

• Myelin membrane encephalitogenic protein antibody

• OTTHUMP00000163776 antibody

• OTTHUMP00000174387 antibody

• OTTHUMP00000174388 antibody

• SHI antibody

• Shiverer antibody

• SP antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody [12] (ab7349)

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labelling Myelin Basic Protein with ab7349 at a 1/200 dilution, followed by Goat anti-Rat secondary (Alexa Fluor® 488) (ab150157) secondary antibody at a 1/1000 dilution. Confocal image showing positive staining in mouse primary oligodendroglia cells (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

  Counterstained with Anti-MAP2 antibody (ab183830) at a 1/1000 dilution, followed by Goat Anti-Rabbit secondary (Alexa Fluor® 594) (ab150080) at a 1/1000 dilution (shown in magenta).

  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  The negative controls are as follows:
  -ve control 1: Myelin Basic Protein (ab7349) at a 1/200 dilution, followed by Goat anti-Rabbit secondary (Alexa Fluor® 594) (ab150080) at a 1/1000 dilution. 
  -ve control 2: Anti-MAP2 (ab183830) at a 1/1000 dilution, followed by Goat anti-Rat secondary (Alexa Fluor® 488) (ab150157) at a 1/1000 dilution.

Western blot - Anti-Myelin Basic Protein antibody [12] (ab7349)

Anti-Myelin Basic Protein antibody [12] (ab7349) at 1/5000 dilution + Human cerebellum tissue lysate at 10 µg

Secondary
Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

Developed using the ECL technique.

Observed band size: 18.5 kDawhy is the actual band size different from the predicted?


Exposure time: 1 second

• 

  Western blot - Anti-Myelin Basic Protein antibody [12] (ab7349)

  All lanes : Anti-Myelin Basic Protein antibody [12] (ab7349) at 1/5000 dilution
  
  Lane 1 : Mouse brain tissue lysate
  Lane 2 : Rat brain tissue lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
  
  Observed band size: 14-21.5 kDawhy is the actual band size different from the predicted?
  
  
  
  

  The molecular weight observed was in consistency with the literature (PMID: 9299539).

  Blocking buffer: 5% NFDM/TBST.

  Exposure time: Lane 1: 3 seconds; Lane 2: 1 second.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] (ab7349)

  Immunohistochemical analysis of paraffin-embedded Human cerebrum labeling Myelin Basic Protein with ab7349 at 1/4000 dilution (0.259 µg/ml) followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer; ab214882). The section was incubated with ab7349 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] (ab7349)

  Immunohistochemical analysis of paraffin-embedded Human cerebrum labeling Myelin Basic Protein with ab7349 at 1/4000 dilution (0.259 µg/ml) followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer; ab214882). The section was incubated with ab7349 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with DAPI.

  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.