Anti-Robo1抗体
参阅全部 Robo1 一抗
兔多克隆抗体to Robo1
Rabbit
Some batches may cross-react with Robo2 and Robo3. Please see abreview for more details.
适用于: ELISA, IHC-P, WB, IHC-Frmore details
与反应: Mouse, Human
Synthetic peptide:
C-VLGGYERGEDNNE
conjugated to KLH, corresponding to amino acids 1632-1644 of Human Robo1.
WB: HEK-293, HeLa, MCF-7, A-549, and A-172 lysates. IHC-P: Human brain tissue. IHC-Fr: Mouse lung tissue.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
Immunogen affinity purified
This affinity purified antibody is directed against human ROBO-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
多克隆
IgG
Abpromise™承诺保证使用ab7279于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | 1/30000 - 1/60000. | |
| IHC-P | (3) | Use a concentration of 2 - 10 µg/ml. |
| WB | (4) | 1/500 - 1/3000. Detects a band of approximately 181 kDa. |
| IHC-Fr | (4) | Use a concentration of 2 - 10 µg/ml. |
Entrez Gene: 6091 Human
Entrez Gene: 19876 Mouse
Omim: 602430 Human
SwissProt: Q9Y6N7 Human
SwissProt: O89026 Mouse
Unigene: 13640 Human
Unigene: 310772 Mouse
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Western blot - Anti-Robo1 antibody (ab7279)
Anti-Robo1 antibody (ab7279) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Robo1 antibody (ab7279)
This image shows staining of human brain tissue.
Immunohistochemistry (Frozen sections) - Anti-Robo1 antibody (ab7279)
Immunohistochemistry analysis of mouse lung tissue sections (adult, frozen 100 µm wholemount sections) labelling Robo1 with ab7279 at 1/50 dilution. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4°C.
Western blot - Anti-Robo1 antibody (ab7279)This image is courtesy of an anonymous Abreview.
Anti-Robo1 antibody (ab7279) at 1/1000 dilution + HEK 293T cells at 25 µg
Developed using the ECL technique.
Performed under reducing conditions.
Western blot - Anti-Robo1 antibody (ab7279)
All lanes : Anti-Robo1 antibody (ab7279) at 1 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 6 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 7 : LNCaP (human prostate cancer cell line) whole cell lysate
Lane 8 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 9 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lane 10 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 11 : A-172 (human brain glioblastoma) whole cell lysate
Lane 12 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Peroxidase Rabbit secondary antibody for 60 minutes at room temperature at 1/30000 dilution
Observed band size: 181 kDawhy is the actual band size different from the predicted?
Primary antibody incubated overnight at 4C.
Blocking Buffer: 1% Casein-TTBS for 30 min at room temperature.
ELISA - Anti-Robo1 antibody (ab7279)
ELISA results of purified ab7279 tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated secondary and TMB ELISA Peroxidase Substrate.
