Anti-Integrin beta 1 antibody [BV7]

• 产品名称

  Anti-Integrin beta 1抗体[BV7]
  参阅全部 Integrin beta 1 一抗

• 描述

  小鼠单克隆抗体[BV7] to Integrin beta 1

• 宿主

  Mouse

• 特异性

  This monoclonal antibody binds to human beta 1 integrin chain present on tumor cells. The antibody is active on HT-29 colon carcinoma cells and on HCCP-2998 tumor cells, as well as several other tumor cells (MG3 osteosarcoma, A375 melanoma, MHCC-1410 and Lovo colon carcinoma) but does not affect adhesion to endothelial cells.

• 经测试应用

  适用于: Flow Cyt (Intra)more details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus corresponding to Human Integrin beta 1. Human umbilical vein EC

• 表位

  Antibody BV7 stains the extracellular domain of beta-1 integrin.

• 阳性对照

• HT-29 colon carcinoma, Endothelial cells. Flow Cyt (Intra): HUVEC cells; MCF-7 cells

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.60
  Constituents: PBS, 0.1% BSA

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  BV7

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab7168于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3688 Human

• Omim: 135630 Human

• SwissProt: P05556 Human

• Unigene: 643813 Human

• 别名

• Fibronectin receptor subunit beta antibody

• FNRB antibody

• Glycoprotein IIa antibody

• GP IIa antibody

• GPIIA antibody

• Integrin beta-1 antibody

• Integrin subunit beta 1 antibody

• integrin VLA-4 beta subunit antibody

• Integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) antibody

• ITB1_HUMAN antibody

• ITGB1 antibody

• MDF2 antibody

• MSK12 antibody

• OTTHUMP00000019420 antibody

• very late activation protein antibody

• Very late activation protein, beta polypeptide antibody

• VLA BETA antibody

• VLA-4 subunit beta antibody

• VLA-BETA antibody

• VLAB antibody

• VLAbeta antibody

• beta1 integrin antibody

• CD_antigen=CD29 antibody

• CD29 antibody

• 

  Flow Cytometry (Intracellular) - Anti-Integrin beta 1 antibody [BV7] (ab7168)

  Overlay histogram showing MCF-7 cells stained with ab7168 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7168, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.
  
  Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

• 

  Flow Cytometry (Intracellular) - Anti-Integrin beta 1 antibody [BV7] (ab7168)

  Overlay histogram showing HUVEC cells stained with ab7168 (blue line). Red line was isotype control mouse IgG₁. Black line was an irrelevant mouse IgG₁ used under the same conditions. Controls and anti-human ß1-integrin at 0.4 µg/100000 cells (1 hr 4°C).