山羊抗小鼠IgG H&L (Alkaline Phosphatase)预吸附二抗
参阅全部 IgG 二抗
Goat
Mouse
No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
适用于: ICC/IF, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, Dot blot, WBmore details
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheepmore details
Full length native mouse IgG (purified).
Alkaline Phosphatase
Liquid
Shipped at 4°C. Store at +4°C.
pH: 8
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), 0.788% Tris HCl, 0.88% Sodium chloride, 0.0095% Magnesium chloride, 0.0014% Zinc chloride
浓度
500 µg 浓度为 1 mg/ml
Affinity purified
Mouse Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
多克隆
Abpromise™承诺保证使用ab7069于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/200 - 1/1000. | |
| ELISA | 1/7000. | |
| IHC-P | (1) | Use at an assay dependent dilution. |
| IHC-Fr | Use at an assay dependent dilution. | |
| Immunomicroscopy | Use at an assay dependent dilution. | |
| Dot blot | Use at an assay dependent dilution. | |
| WB | 1/1000 - 1/2000. |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) preadsorbed (ab7069)This image is courtesy of an anonymous Abreview.
ab13918 staining RANK in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA/5% normal goat serum/0.1% saponin for 30 minutes at 22°C. Samples were incubated with primary antibody (1/50 in blocking buffer) for 9 hours at 4°C. An alkaline phosphatase-conjugated goat anti-mouse IgG H&L (ab7069) (1/300) was used as the secondary antibody.
