山羊抗小鼠IgG H&L (FITC)预吸附二抗
参阅全部 IgG 二抗
Goat
Mouse
No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
适用于: WB, Dot blot, ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheepmore details
Full length native Mouse IgG (purified).
FITC. Ex: 493nm, Em: 528nm
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
500 µg 浓度为 1 mg/ml
Affinity purified
Goat Anti-Mouse IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
多克隆
IgG
Abpromise™承诺保证使用ab7064于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 36 kDa. | |
| Dot blot | Use at an assay dependent concentration. | |
| ICC/IF | (1) | Use at an assay dependent concentration. PubMed: 15767251 |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| Flow Cyt | (1) | 1/1000 - 1/2000. |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | (1) | Use at an assay dependent concentration. |
| ELISA | 1/10000 - 1/50000. |
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064)Humphries, W.H. 4th et al PLoS One. 2011;6(10):e26626. doi: 10.1371/journal.pone.0026626. Epub 2011 Oct 24 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Rab7 and LAMP1 are highly colocalized in BS-C-1 cells and in HeLa cells
LAMP1 was detected with a mouse antiLAMP1 antibody in ICC/IF analysis of BS-C-1 cells.
Anti-LAMP1 was visualized using ab7064 at 1/500 diulution.
Flow Cytometry - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064)
Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (FITC, pre-adsorbed) (ab7064) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064)
ab7064 was used at dilution 1/20 with the primary antibody ab7852 in ICC. See the review on ab7852.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) preadsorbed (ab7064)
ab7064 was used at dilution 1/2000 with the primary antibody ab2378 in IHC-P. See the review on ab2378.
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