Anti-Collagen V抗体
参阅全部 Collagen V 一抗
兔多克隆抗体to Collagen V
Rabbit
Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
适用于: WB, Sandwich ELISA, IHC-P, IP, IHC-Fr, ICCmore details
与反应: Mouse, Cow, Human
Full length native protein (purified) corresponding to Collagen V aa 1-1745.
Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
Immunogen affinity purified
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
多克隆
IgG
Abpromise™承诺保证使用ab7046于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/5000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa). Native conditions are recommended. | |
| Sandwich ELISA | Use at an assay dependent concentration. Can be used with a suitable biotinylated detection antibody such as Rabbit polyclonal to Collagen V (Biotin) (ab6582). | |
| IHC-P | (6) | 1/50 - 1/200. |
| IP | Use at an assay dependent concentration. | |
| IHC-Fr | (2) | Use at an assay dependent concentration. |
| ICC | Use at an assay dependent concentration. PubMed: 18385800 |
Entrez Gene: 1289 Human
Entrez Gene: 1290 Human
Entrez Gene: 50509 Human
Entrez Gene: 12831 Mouse
Entrez Gene: 12832 Mouse
Entrez Gene: 53867 Mouse
Omim: 120190 Human
Omim: 120215 Human
Omim: 120216 Human
SwissProt: P05997 Human
SwissProt: P20908 Human
SwissProt: P25940 Human
SwissProt: Q15094 Human
SwissProt: Q53WR4 Human
SwissProt: Q5PR22 Human
SwissProt: Q5SY11 Human
SwissProt: Q86XF6 Human
SwissProt: O88207 Mouse
SwissProt: Q3U962 Mouse
SwissProt: Q920S4 Mouse
Unigene: 210283 Human
Unigene: 7281 Mouse
AB collagen antibody
Alpha 1 type V collagen antibody
Alpha 2 type V collagen antibody
CO5A1_HUMAN antibody
COL5A1 antibody
Col5A2 antibody
COL5A2 protein antibody
Col5A3 antibody
Collagen alpha 1(V) chain antibody
Collagen alpha 2 (V) chain precursor antibody
Collagen alpha 2(V) chain antibody
Collagen alpha 3(V) chain antibody
Collagen alpha-1(V) chain antibody
Collagen fetal membrane A polypeptide antibody
Collagen type V alpha 1 antibody
Collagen type V alpha 2 antibody
Collagen type V alpha 3 antibody
Collagen V alpha 2 polypeptide antibody
CollagenV antibody
MGC105115 antibody
OTTHUMP00000064637 antibody
Pro alpha 1 type V collagen antibody
Pro alpha 3(V) collagen antibody
Procollagen alpha 2(V) antibody
Type V preprocollagen alpha 2 chain antibody
Western blot - Anti-Collagen V antibody (ab7046)
Anti-Collagen V antibody (ab7046) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 180 kDa
Observed band size: 180 kDa
Additional bands at: 122 kDa, 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (ab7046)This image is courtesy of an anonymous Abreview.
ab7046 staining Collagen V in Human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate pH 6 prior to blocking with 0.25% casein for 5 minutes at 25°C. The primary antibody was diluted 1/75 in Tris--HCL and incubated with the sample for 30 minutes at 25°C. An HRP polymer-conjugated goat anti-rabbit antibody was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (ab7046)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung (left) and placenta (right) sections labelling collagen V with ab7046 at a dilution of 1/200 for 45 minutes at room temperature. An antigen retrival step was performed with 0.01 M sodium citrate buffer pH 6.0 at 100ºC for 20 mins.
