Anti-HDAC2 antibody

• 产品名称

  Anti-HDAC2抗体
  参阅全部 HDAC2 一抗

• 描述

  兔多克隆抗体to HDAC2

• 宿主

  Rabbit

• 经测试应用

  适用于: IP, ICC/IFmore details

• 种属反应性

  与反应: Human
  预测可用于: Rat, Chicken

• 免疫原

  Synthetic peptide corresponding to Human HDAC2 aa 471-488 conjugated to Keyhole Limpet Haemocyanin (KLH). The epitope recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding.
  Sequence:

  C-SGEKTDTKGTKSEQLSNP

  Run BLAST with Run BLAST with 

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: 1% BSA

• 浓度

• 50 µl 浓度为 14.3 mg/ml

• 纯度

  IgG fraction

• 纯化说明

  Whole antiserum is fractionated and further purified by anion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab7029于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 395635 Chicken

• Entrez Gene: 3066 Human

• Entrez Gene: 84577 Rat

• Omim: 605164 Human

• SwissProt: P56519 Chicken

• SwissProt: Q92769 Human

• Unigene: 3352 Human

• 别名

• HDAC 2 antibody

• Hdac2 antibody

• HDAC2_HUMAN antibody

• Histone deacetylase 2 (HD2) antibody

• Histone deacetylase 2 antibody

• OTTHUMP00000017046 antibody

• OTTHUMP00000227077 antibody

• OTTHUMP00000227078 antibody

• RPD3 antibody

• transcriptional regulator homolog RPD3 antibody

• YAF1 antibody

• YY1 associated factor 1 antibody

• YY1 transcription factor binding protein antibody

• Yy1bp antibody

• D10Wsu179e antibody

• HD 2 antibody

• HD2 antibody

• 

  Immunoprecipitation - Anti-HDAC2 antibody (ab7029)

  Immunoprecipitation analysis of HeLa whole cell lysates, ab7029 was used to precipitate HDAC3 from solution.

  
  
  Lane 1 : Anti-HDAC2 antibody (ab7029) at 5 µl
  Lane 2 : Anti-HDAC2 antibody (ab7029) at 10 µl
  Lane 3 : Negative Control
  
  All lanes : HeLa whole cell lysate.
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-HDAC2 antibody (ab7029)This image is courtesy of Michael Mancini, Baylor College of Medicine

  HeLa cells were fixed with 4% formaldehyde in PEM buffer. The coverslip was incubated in blocking buffer of 5% powdered milk in TBS-T plus 0.02% sodium azide for 1 hour at room temperature. Blocking buffer was removed and primary antibody was added at a dilution of 1/600 and incubated overnight at 4 degrees celsius. The coverslips were then washed 4-5 times with blocking buffer for 5 minutes. Secondary antibody, goat anti-rabbit Alexa 594, was added at a dilution of 1/1000 and incubated at room temperature for one hour. From this point on coverslips were covered with foil to protect them from light. They were washed 5 times with TBS-T and then one time with PEM, for 5 minutes each wash. The coverslips were fixed 10-30 minutes in 4% formaldehyde in PEM buffer, then washed 3 times with PEM buffer for 5 minutes. 0.1M ammonium chloride in PEM buffer was added for 10 minutes to quench auto-florescence, and then slips were washed 2 times for 5 minutes in PEM followed by 3 washes for 5 minute

• 

  Immunoprecipitation - Anti-HDAC2 antibody (ab7029)

  HDAC2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to HDAC2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
  The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
  Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7029.
  Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
  Band: Band: 60ka: HDAC2; 55kDa; Heavy Chain.