Anti-HDAC1抗体
参阅全部 HDAC1 一抗
兔多克隆抗体to HDAC1
Rabbit
适用于: ICC/IF, WB, IPmore details
与反应: Mouse, Rat, Human, Chinese hamster
Synthetic peptide corresponding to Human HDAC1 aa 466-482 conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:
C-KEEKPEAKGVKEEVKLA
Database link: HDAC1
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
IgG fraction
Whole antiserum is fractionated and further purified by anion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
多克隆
IgG
Abpromise™承诺保证使用ab7028于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (4) | 1/200. |
| WB | (8) | 1/2000 - 1/20000. Predicted molecular weight: 55 kDa. |
| IP | (1) | Use at an assay dependent concentration. Use 5 -10 μL antibody per reaction |
Entrez Gene: 3065 Human
Entrez Gene: 433759 Mouse
Omim: 601241 Human
SwissProt: Q13547 Human
SwissProt: O09106 Mouse
Unigene: 88556 Human
Unigene: 202504 Mouse
Unigene: 391033 Mouse
Unigene: 1863 Rat
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Western blot - Anti-HDAC1 antibody - ChIP Grade (ab7028)
All lanes : Anti-HDAC1 antibody (ab7028) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
Lane 4 : CHO (Chinese hamster ovary cell line) cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Secondary
All lanes : Goat Anti-Rabbit HRP
Predicted band size: 55 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody (ab7028)Hanigan, T.W. et al Send to PLoS One. 2017 Oct 18;12(10):e0186620. doi: 10.1371/journal.pone.0186620. eCollection 2017 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
MCF-7 cells were treated with indicated concentrations of panobinostat (optical sections A-D, respectively) or trichostatin A (optical sections E-H, respectively) for 12 hours, fixed, permeabilized and optical sections were obtained by laser scanning confocal microscopy. Fluorescence signal for HDAC1 is shown in green (left panels), DAPI staining is shown in blue (middle panels), and merged optical sections are shown in the right panels. Colocalization analysis of HDAC1 fluorescence signal and the DAPI stain signal was performed with JACoP (ImageJ).
HDAC1 was detected with ab7028. cells were ficed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
(After Figure 3 of Hanigan et al).
Immunoprecipitation - Anti-HDAC1 antibody - ChIP Grade (ab7028)
HDAC1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with ab7028.
Lane 1: 5 μl ab7028 in HeLa whole cell lysate.
Lane 2: 10 μl ab7028 in HeLa whole cell lysate.
Lane 3: 10 μl control antibody in HeLa whole cell lysate.
Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody (ab7028)
Immunofluorescence analysis of methanol fixed HeLa cells with ab7028 labelling HDAC1 at 1/200 dilution. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
Western blot - Anti-HDAC1 antibody - ChIP Grade (ab7028)This image is courtesy of an anonymous Abreview
All lanes : Anti-HDAC1 antibody (ab7028) at 1/1000 dilution
Lane 1 : Whole cell lysate from human HEK293 cell line
Lane 2 : Whole cell lysate from human HEK293 cell line treated with HDAC1 gene silencing shRNA
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit Ig at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Exposure time: 10 seconds
Immunoprecipitation - Anti-HDAC1 antibody - ChIP Grade (ab7028)
HDAC1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to HDAC1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7028.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 60ka: HDAC1.
Western blot - Anti-HDAC1 antibody - ChIP Grade (ab7028)This image is courtesy of an anonymous Abreview
Anti-HDAC1 antibody (ab7028) at 1/2000 dilution + Human HuH-7 whole cell lysate at 15 µg
Secondary
HRP conjugated Sheet anti-rabbit IgG polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Exposure time: 20 seconds
Blocked with 5% milk for 1 hour at 20°C
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