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Anti-PRMT1 antibody [MAT-B12]

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  • 产品名称

    Anti-PRMT1抗体[MAT-B12]
    参阅全部 PRMT1 一抗

  • 描述

    小鼠单克隆抗体[MAT-B12] to PRMT1

  • 宿主

    Mouse

  • 特异性

    This Mab reacts with human arginine N-Methyl Transferase

  • 经测试应用

    适用于: Flow CytICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Fusion protein corresponding to Human PRMT1.
    Database link: 
    Q99873

  • 阳性对照

    • Tested on HELA whole cell extract and transfected Cos cells (recombinant protein PRMT1). The results showed good reactivity of the antibody in both cases. ICC/IF - MCF-7 cells

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose

  • 浓度

    • 100 µg 浓度为 1 mg/ml

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    MAT-B12

  • 同种型

    IgG1

  • 轻链类型

    kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab7027于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
Flow Cyt

Use 2µg for 106 cells. 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF

Use at an assay dependent concentration.

  • 数据库链接

  • 别名

    • HCP 1 antibody

    • HCP1 antibody

    • Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2 antibody

    • Heterogeneous nuclear ribonucleoproteins methyltransferase like 2 antibody

    • Heterogeneous nuclear ribonucleoproteins methyltransferase like2 antibody

    • Histone-arginine N-methyltransferase PRMT1 antibody

    • HMT 2 antibody

    • HMT1 (hnRNP methyltransferase S. cerevisiae) like 2 antibody

    • HMT1 hnRNP methyltransferase antibody

    • HMT1 hnRNP methyltransferase like 2 (S. cerevisiae) antibody

    • HMT1 hnRNP methyltransferase like 2 antibody

    • HMT2 antibody

    • HRMT1 L2 antibody

    • HRMT1L 2 antibody

    • HRMT1L2 antibody

    • Human mRNA for suppressor for yeast mutant antibody

    • Human mRNA for suppressor for yeast mutant complete cds antibody

    • Interferon receptor 1 bound protein 4 antibody

    • Interferon receptor 1 bound protein4 antibody

    • Interferon receptor 1-bound protein 4 antibody

    • Interferon receptor 1bound protein 4 antibody

    • IR1 B4 antibody

    • IR1B 4 antibody

    • IR1B4 antibody

    • Mrmt 1 antibody

    • Mrmt1 antibody

    • PRMT 1 antibody

    • PRMT1 antibody

    • Protein arginine methyltransferase 1 antibody

    • Protein arginine N methyltransferase 1 antibody

    • Protein arginine N methyltransferase1 antibody

    • Protein arginine N-methyltransferase 1 antibody

    • R1B4 antibody

    • S. cerevisiae like 2 antibody

    • ANM 1 antibody

    • ANM1 antibody

    • ANM1_HUMAN antibody

  • Immunocytochemistry/ Immunofluorescence - Anti-PRMT1 antibody [MAT-B12] (ab7027)

    Immunocytochemistry/ Immunofluorescence - Anti-PRMT1 antibody [MAT-B12] (ab7027)

    Immunofluorecent analysis of MCF-7 cells labelling PRMT1 with ab7027

  • Flow Cytometry - Anti-PRMT1 antibody [MAT-B12] - ChIP Grade (ab7027)

    Flow Cytometry - Anti-PRMT1 antibody [MAT-B12] - ChIP Grade (ab7027)

    Overlay histogram showing HeLa cells stained with ab7027 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7027, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.