Anti-PRMT1抗体[MAT-B12]
参阅全部 PRMT1 一抗
小鼠单克隆抗体[MAT-B12] to PRMT1
Mouse
This Mab reacts with human arginine N-Methyl Transferase
适用于: Flow Cyt, ICC/IFmore details
与反应: Human
Fusion protein corresponding to Human PRMT1.
Database link: Q99873
Tested on HELA whole cell extract and transfected Cos cells (recombinant protein PRMT1). The results showed good reactivity of the antibody in both cases. ICC/IF - MCF-7 cells
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
浓度
100 µg 浓度为 1 mg/ml
Protein A purified
单克隆
MAT-B12
IgG1
kappa
Abpromise™承诺保证使用ab7027于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt | Use 2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. | |
ICC/IF | Use at an assay dependent concentration. |
Entrez Gene: 3276 Human
Omim: 602950 Human
SwissProt: Q99873 Human
Unigene: 20521 Human
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Immunocytochemistry/ Immunofluorescence - Anti-PRMT1 antibody [MAT-B12] (ab7027)
Immunofluorecent analysis of MCF-7 cells labelling PRMT1 with ab7027
Flow Cytometry - Anti-PRMT1 antibody [MAT-B12] - ChIP Grade (ab7027)
Overlay histogram showing HeLa cells stained with ab7027 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7027, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.