Anti-Adenovirus Type 5抗体
兔多克隆抗体to Adenovirus Type 5
Rabbit
适用于: Neutralising, ELISA, WB, ICC/IFmore details
与反应: Human adenovirus 5
Tissue, cells or virus corresponding to Adenovirus Type 5. Purified Adenovirus type 5
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab6982于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Neutralising | Use at an assay dependent concentration. This antibody will neutralize Ad5 infection in a bioassay (1/25,000 dilution for 50 % inactivation of Ad5at 1000 particles/ml) - PMID: 14605137 | |
| ELISA | (1) | Use at an assay dependent concentration. Recognizes Ad5 immobilized on microtiter plates. |
| WB | (5) | Use at an assay dependent concentration. |
| ICC/IF | (1) | Use at an assay dependent concentration. |
Adenovirus 5 antibody
AdV 5 antibody
Immunocytochemistry/ Immunofluorescence - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous Abreview.
ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4°C. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 546 (red) used at a 1/1000 dilution.
Western blot - Anti-Adenovirus Type 5 antibody (ab6982)
Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte).
Bottom blot (B): As above, except ab6982 omitted.
Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte). Bottom blot (B): As above, except ab6982 omitted.
Western blot - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous Abreview
All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution
All lanes : human hepatocytes whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit secondary polyclonal antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 130 kDawhy is the actual band size different from the predicted?
Exposure time: 20 seconds
Blocked with 5% milk
Western blot - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous Abreview.
All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution
Lane 1 : Whole cell lysate prepared from U2OS cells.
Lane 2 : Whole cell lysate prepared from U2OS cells infected with Adenovirus 5.
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Goat anti-rabbit conjugated to IRDye 700DX
Blocking step was performed using 5% milk for 1 hour at 23°C.
