Anti-Thrombomodulin antibody [PBS-01]

• 产品名称

  Anti-Thrombomodulin抗体[PBS-01]
  参阅全部 Thrombomodulin 一抗

• 描述

  小鼠单克隆抗体[PBS-01] to Thrombomodulin

• 宿主

  Mouse

• 经测试应用

  适用于: ELISA, WB, Flow Cyt, IHC-Pmore details

• 种属反应性

  与反应: Rabbit, Human
  不与反应: Pig

• 免疫原

  Recombinant full length protein corresponding to Human Thrombomodulin.
  Database link: P07204

• 表位

  EGF domain 5 of thrombomodulin.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 6.50
  Constituents: 0.292% Sodium chloride, 4% Sucrose, 0.1% Tween, 0.41% Sodium acetate

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  PBS-01

• 骨髓瘤

  Sp2

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab6980于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 7056 Human

• Omim: 188040 Human

• SwissProt: P07204 Human

• Unigene: 2030 Human

• 别名

• BDCA3 antibody

• CD 141 antibody

• CD141 antibody

• CD141 antigen antibody

• Fetomodulin antibody

• Thbd antibody

• THPH12 antibody

• THRM antibody

• Thrombomodulin antibody

• TM antibody

• TRBM_HUMAN antibody

• AHUS 6 antibody

• AHUS6 antibody

• BDCA 3 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-01] (ab6980)

  Ab6980 staining human normal lung endothelium. Staining is localized to the membrane.
  Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

• 

  Flow Cytometry - Anti-Thrombomodulin antibody [PBS-01] (ab6980)

  Overlay histogram showing THP1 cells stained with ab6980 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6980, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.