驴抗山羊IgG H&L (Cy3 ®)预吸附二抗
参阅全部 IgG 二抗
Donkey
Goat
适用于: ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA, WBmore details
Cow, Guinea pig, Hamster, Horse, Mouse, Rabbit, Ratmore details
Full length native Goat IgG (purified).
Cy3 ®. Ex: 552nm, Em: 565nm
Liquid
Shipped at 4°C. Store at +4°C.
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
浓度
500 µg 浓度为 1 mg/ml
Immunogen affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
Cy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 8.1 moles Cy3 per mole of Donkey IgG
多克隆
IgG
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
Abpromise™承诺保证使用ab6949于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/1000 - 1/5000. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | (1) | Use at an assay dependent concentration. |
| ICC | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. | |
| ELISA | 1/10000 - 1/50000. | |
| WB | 1/1000. Predicted molecular weight: 36 kDa. |
Western blot - Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949)
Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949) at 1000 cells + Goat IgG, 50 ng
Predicted band size: 36 kDa
Both the heavy and light chains of Goat IgG are observed.
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