山羊抗小鼠IgG H&L (Cy2 ®)预吸附二抗
参阅全部 IgG 二抗
Goat
Mouse
适用于: ICC/IF, IHC-P, ELISA, WB, Flow Cyt, IHC-Frmore details
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheepmore details
Full length native protein (purified) corresponding to IgG.
Cy2 ®. Ex: 489nm, Em: 505nm
Liquid
Shipped at 4°C. Store at +4°C.
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
Immunogen affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 5.1 moles Cy2 per mole of Goat IgG
多克隆
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
Abpromise™承诺保证使用ab6944于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | 1/1000 - 1/5000. | |
IHC-P | Use at an assay dependent concentration. | |
ELISA | 1/10000 - 1/50000. | |
WB | Use at an assay dependent concentration. | |
Flow Cyt | Use at an assay dependent concentration. | |
IHC-Fr | Use at an assay dependent concentration. |
Western blot - Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed (ab6944)
Secondary
Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed (ab6944) at 1/1000 dilution (60 minutes at Room temperature)
Observed band size: 25, 55 kDawhy is the actual band size different from the predicted?