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Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed

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500ug
- +
产品详情
  • 产品名称

    山羊抗小鼠IgG H&L (Cy2 ®)预吸附二抗
    参阅全部 IgG 二抗

  • 宿主

    Goat

  • 靶标种属

    Mouse

  • 经测试应用

    适用于: ICC/IFIHC-PELISAWBFlow CytIHC-Frmore details

  • 最小交叉反应


    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Rabbit, Rat, Sheepmore details

  • 免疫原

    Full length native protein (purified) corresponding to IgG.

  • 偶联物

    Cy2 ®. Ex: 489nm, Em: 505nm

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 6.50
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA

  • 纯度

    Immunogen affinity purified

  • 纯化说明

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

  • 共轭说明

    Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 5.1 moles Cy2 per mole of Goat IgG

  • 克隆

    多克隆

  • 常规说明

    Cy™ and CyDye™ are registered trademarks of Cytiva.

    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

The Abpromise guarantee

Abpromise™承诺保证使用ab6944于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
ICC/IF

1/1000 - 1/5000.

IHC-P

Use at an assay dependent concentration.

ELISA

1/10000 - 1/50000.

WB

Use at an assay dependent concentration.

Flow Cyt

Use at an assay dependent concentration.

IHC-Fr

Use at an assay dependent concentration.

Western blot - Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed (ab6944)

Western blot - Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed (ab6944)

Secondary
Goat Anti-Mouse IgG H&L (Cy2 ®) preadsorbed (ab6944) at 1/1000 dilution (60 minutes at Room temperature)

Observed band size: 25, 55 kDa
why is the actual band size different from the predicted?