山羊抗兔IgG H&L (Cy2 ®)预吸附二抗
参阅全部 IgG 二抗
Goat
Rabbit
适用于: WB, ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheepmore details
Full length native Rabbit IgG (purified).
Cy2 ®. Ex: 489nm, Em: 505nm
Liquid
Shipped at 4°C. Store at +4°C.
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA
Immunogen affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 2.0 moles Cy2 per mole of Goat IgG
多克隆
IgG
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
Abpromise™承诺保证使用ab6940于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use at an assay dependent dilution. | |
| ICC/IF | 1/1000 - 1/5000. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC | (1) | Use at an assay dependent concentration. |
| Flow Cyt | Use at an assay dependent dilution. | |
| ELISA | 1/10000 - 1/50000. |
Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)
Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution + Rabbit IgG at 0.05 µg
Incubated with ab6940 for 1 hour at room temperature.
Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)
All lanes : Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution
Lane 1 : Rabbit IgG whole molecule
Lane 2 : Rabbit IgG F(ab) fragment
Lane 3 : Rabbit IgG F(c) fragment
Lane 4 : Rabbit IgM whole molecule
Lane 5 : Normal rabbit serum
Lysates/proteins at 0.05 µg per lane.
Secondary
All lanes : Peroxidase-conjugated donkey anti-goat IgG at 1/40000 dilution
Performed under reducing conditions.
Observed band size: 23,25,50,70 kDawhy is the actual band size different from the predicted?
Incubated with the primary antibody for 1 hour at room temperature.
Incubated with the secondary antibody for 30 minutes at room temperature.
Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)
ab6940 was used at dilution 1/10000 with the primary antibody ab47840 in WB. See the review on ab47840.
