驴抗羊IgG H&L (HRP)
参阅全部 IgG 二抗
Donkey
Sheep
适用于: ICC/IF, IHC-Fr, IHC-P, WB, ELISA, Immunomicroscopy, Dot blotmore details
Full length native Sheep IgG (purified).
HRP
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
Affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads.
Horseradish Peroxidase (HRP)
多克隆
IgG
Abpromise™承诺保证使用ab6900于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/1000 - 1/5000. | |
| IHC-Fr | 1/1000 - 1/5000. | |
| IHC-P | 1/1000 - 1/5000. | |
| WB | 1/3000 - 1/15000. | |
| ELISA | 1/150000. | |
| Immunomicroscopy | Use at an assay dependent dilution. | |
| Dot blot | Use at an assay dependent dilution. |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)
IHC image of Histone H3 staining in human normal colon formalin fixed paraffin embedded tissue section*.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab128012, 10 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6900, 1/1000 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)
ab6900 was used at dilution 1/20000 with the primary antibody ab30524 in WB. See the review on ab30524.
ELISA - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)
ELISA results of purified ab6900 tested against purified Sheep IgG.
Each well was coated in duplicate with 1.0 µg of Sheep IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
