山羊抗鸡IgY H&L (Biotin)
参阅全部 IgY 二抗
Goat
Chicken
适用于: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
chicken IgY whole molecule
Biotin
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
1 mg 浓度为 2 mg/ml
Affinity purified
Anti-Chicken IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per IgG molecule
多克隆
IgG
Abpromise™承诺保证使用ab6876于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | 1/500 - 1/3000. | |
| Dot blot | Use at an assay dependent concentration. | |
| ELISA | 1/98500. | |
| IHC-P | (1) | Use at an assay dependent concentration. |
| IHC-Fr | Use at an assay dependent concentration. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| WB | 1/3000 - 1/30000. |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)This image is courtesy of an Abreview submitted by Carl Hobbs.
ab89999 staining GFAP in rat spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 22°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/300 in TBS/BSA/Azide) for 2 hours at 22°C. A biotin-conjugated goat anti-chicken IgY H&L (ab6876) (1/100) was used as the secondary antibody.
Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)Image from Yang Z et al., J Biol Chem. 2011 Apr 1;286(13):11444-55. Epub 2011 Jan 29. Fig 3.; doi: 10.1074/jbc.M110.212480; April 1, 2011, The Journal of Biological Chemistry, 286, 11444-11455.
Immunohistochemical analyis of Human lung tissue stained with a chicken primary antibody, followed by ab6876 at 1/500 dilution and horseradish peroxidase-conjugated streptavidin. Staining was detected using DAB.
