山羊抗大鼠IgG H&L (Biotin)
参阅全部 IgG 二抗
Goat
Rat
适用于: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
Rat IgG whole molecule
Biotin
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
1 mg 浓度为 2 mg/ml
Affinity purified
Anti-RAT IgG Biotin Conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
多克隆
Abpromise™承诺保证使用ab6844于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/2000 - 1/10000. | |
ELISA | Use at an assay dependent concentration. | |
Immunomicroscopy | Use at an assay dependent concentration. | |
Dot blot | Use at an assay dependent concentration. | |
ICC/IF | 1/1000 - 1/5000. | |
IHC-P | Use at an assay dependent concentration. | |
IHC-Fr | Use at an assay dependent concentration. |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (Biotin) (ab6844)
Ab6844 was used at dilution 1/500 with the primary antibody ab6640 in IHC-P. See the review on ab6640.