Donkey Anti-Mouse IgG H&L (HRP)

• 产品名称

  驴抗小鼠IgG H&L (HRP)
  参阅全部 IgG 二抗

• 宿主

  Donkey

• 靶标种属

  Mouse

• 经测试应用

  适用于: Dot blot, ELISA, IHC-P, IHC-Fr, WB, ICC/IFmore details

• 免疫原

  mouse IgG whole molecule

• 偶联物

  HRP

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C.

• 存储溶液

  Preservative: 0.01% Gentamicin sulphate
  Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride

• 纯度

  Affinity purified

• 纯化说明

  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.

• 共轭说明

  Horseradish Peroxidase (HRP)

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab6820于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 

  Western blot - Donkey Anti-Mouse IgG H&L (HRP) (ab6820)Portegijs et al PLoS Genet. 2016 Oct 6;12(10):e1006291. doi: 10.1371/journal.pgen.1006291. eCollection 2016 Oct. Fig S5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Detection of protein levels in LIN-5 mutants.

  (Panel A) Western blots of lysates of yeast clones containing the indicated LIN-5 expression constructs, probed for LIN-5 and tubulin (loading control) levels.

  Protein samples were separated on gradient acrylamide gels and subjected to western blotting on polyvinylidene difluoride membrane. Membranes were blocked with 5% skim milk in PBST for 1 hour at room temperature, or overnight at 4°C for stripped blots. For protein detection, primary antibodies used in this study were: mouse anti–LIN-5 (1:1000) and rabbit anti-Tubulin (1:1000, Abcam) for stripped blots. Secondary antibodies used were: donkey anti-mouse HRP (1:5000, Abcam, ab6820) and goat anti-rabbit HRP (1:5000).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (HRP) (ab6820)

  IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*.

  The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8224, 3 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6820, 1/2000 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

  The inset negative control image is secondary-only at 1/500 dilution.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre