山羊抗小鼠IgG H&L (FITC)
参阅全部 IgG 二抗
Goat
Mouse
适用于: Dot blot, IHC (PFA fixed), Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA, WBmore details
mouse whole molecule
FITC. Ex: 493nm, Em: 528nm
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.01% BSA, 0.424% Potassium phosphate solution, 50% Glycerol (glycerin, glycerine)
浓度
1 mg 浓度为 2 mg/ml
Affinity purified
Goat Anti-Mouse IgG [H&L] FITC Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles FITC per mole of Goat IgG
多克隆
IgG
Abpromise™承诺保证使用ab6785于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot | Use at an assay dependent concentration. | |
| IHC (PFA fixed) | 1/1000. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| Flow Cyt | 1/500 - 1/2500. PubMed: 17989178 | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 15716365For PFA fixed tissue see PMID 15716365. | |
| ICC/IF | (1) | 1/1000 - 1/5000. (PMID16006199) |
| ELISA | 1/10000 - 1/50000. | |
| WB | 1/10000. PubMed: 17217624 |
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (ab6785)This image is courtesy of an Abreview submitted by J Chai.
ab8978 staining vimentin in human colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton X-100 and blocked with Dako serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in Dako antibody diluent) for 2 hours at 28°C. A FITC-conjugated goat anti-mouse IgG H&L (ab6785) (1/800) was used as the secondary antibody.
Flow Cytometry - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
Ab6785 was used at dilution 1/100 with the primary antibody ab52455 in Flow Cyt. See the review on ab52455.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
Ab6785 was used at dilution 1/2000 with the primary antibody ab1220 in ICC/IF. See the review on ab1220.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) (ab6785)
Ab6785 was used at dilution 1/300 with the primary antibody ab6002 in IHC-P. See the review on ab6002.
