兔抗人IgG H&L (HRP)
参阅全部 IgG 二抗
Rabbit
Human
适用于: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF, Competitive ELISAmore details
human IgG whole molecule
HRP
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
1 mg 浓度为 2 mg/ml
Affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads.
Horseradish Peroxidase (HRP)
多克隆
IgG
Abpromise™承诺保证使用ab6759于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot | Use at an assay dependent dilution. | |
| ELISA | (1) | 1/130000. |
| IHC-P | Use at an assay dependent dilution. | |
| IHC-Fr | Use at an assay dependent dilution. | |
| Immunomicroscopy | Use at an assay dependent dilution. | |
| WB | 1/5000 - 1/25000. | |
| ICC/IF | 1/1000 - 1/5000. | |
| Competitive ELISA | Use at an assay dependent dilution. PubMed: 20573830 |
ELISA - Rabbit Anti-Human IgG H&L (HRP) (ab6759)
ELISA results of ab6759 tested against purified human IgG protein.
Each well was coated in duplicate with 1.0 µg of human IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log 10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
