山羊抗兔IgG H&L (Alkaline Phosphatase)
参阅全部 IgG 二抗
Goat
Rabbit
适用于: IHC-P, WB, ELISA, Dot blot, ICC/IF, IHC-Fr, ICCmore details
Rabbit IgG whole molecule
Alkaline Phosphatase
Liquid
Shipped at 4°C. Store at +4°C.
pH: 8
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), 0.788% Tris HCl, 0.88% Sodium chloride, 0.00952% Magnesium chloride, 0.00136% Zinc chloride
浓度
1 mg 浓度为 1 mg/ml
Affinity purified
Anti-Rabbit IgG (H&L) Antibody Alkaline Phosphatase conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
多克隆
IgG
Abpromise™承诺保证使用ab6722于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/1000 - 1/4000. | |
| ELISA | 1/3000 - 1/25000. | |
| Dot blot | Use at an assay dependent concentration. | |
| ICC/IF | 1/200 - 1/1000. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC | Use at an assay dependent concentration. |
Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
ab6722 was used at dilution 1/10000 with the primary antibody ab16731 in WB. See the review on ab16731.
ELISA - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
ab6722 was used with the primary antibody ab2594 in ELISA. See Abreview on ab2594.
Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody.
Lane M: 3 µl Molecular ladder.
Lane 1: Rabbit IgG whole molecule.
Lane 2: Rabbit IgG F(ab) Fragment.
Lane 3: Rabbit IgG F(c) Fragment.
Lane 4: Rabbit IgM Whole Molecule.
Lane 5: Normal Rabbit Serum.
All samples were reduced. Load: 50 ng per lane. Blocked for 30 min at RT.
Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 for 30 min at RT.
Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM.
Rabbit F(c) migrates slightly higher.
Dot Blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
Dot Blot of ab6722.
Antigen: Rabbit IgG.
Load: Lane 1 - 200ng; Lane 2 - 66.7ng; Lane 3 - 22.2ng; Lane 4 - 7.4ng; Lane 5 - 2.5ng.
Secondary antibody: ab6722 used at 1:1,000 for 60 min at RT. Blocked for 60 min at RT.
Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.
Western blot - Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
Lane 1: Rabbit IgG.
Load: 100 ng per lane.
Secondary antibody: ab6722 used at 1:1,000 for 60 min at RT. Blocked for 30 min at RT.
Predicted/Observed size: 55 and 28 kDa, 55 kDa for Rabbit IgG.
