Goat Anti-Mouse IgG H&L

• 产品名称

  山羊抗小鼠IgG H&L
  参阅全部 IgG 二抗

• 宿主

  Goat

• 靶标种属

  Mouse

• 经测试应用

  适用于: ELISA, IP, Immunodiffusion, Conjugation, ICC/IF, IHC-P, IHC-Fr, WBmore details

• 免疫原

  Mouse IgG, whole molecule

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride

• 纯度

  Affinity purified

• 纯化说明

  Secondary Antibody Anti-Mouse IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab6708于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 

  Western blot - Goat Anti-Mouse IgG H&L (ab6708)

  Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution + Mouse IgG at 0.1 µg
  
  Secondary
  Secondary antibody: DyLight™ 800 goat secondary antibody at 1/20000 dilution
  
  Observed band size: 28,55 kDawhy is the actual band size different from the predicted?
  
  
  
  

  ab6708 incubation overnight at 4°C.

  Both heavy (55kDa) and light (28kDa) chains are detected.

• 

  Western blot - Goat Anti-Mouse IgG H&L (ab6708)

  All lanes : Goat Anti-Mouse IgG H&L (ab6708) at 1/1000 dilution
  
  Lane 1 : Mouse IgG (whole molecule)
  Lane 2 : Mouse IgG Fc fragment
  Lane 3 : Mouse IgG Fab fragment
  Lane 4 : Mouse IgM kappa
  Lane 5 : Mouse serum
  
  Lysates/proteins at 0.05 µg per lane.
  
  Secondary
  All lanes : HRP Donkey Anti-Goat IgG at 1/40000 dilution
  
  
  

  ab6708 incubated for 1 hour at room temperature.