HRP Anti-Fluorescein抗体
参阅全部 Fluorescein 一抗
HRP山羊多克隆抗体to Fluorescein
Goat
HRP
适用于: ELISA, IHC-P, IHC-Fr, WBmore details
与反应: Species independent
Chemical/ Small Molecule corresponding to Fluorescein. Fluorescein conjugated to Goat IgG
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Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1 mg/ml
Affinity purified
Fluorescein Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to sepharose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
多克隆
IgG
Abpromise™承诺保证使用ab6656于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | 1/200000. | |
| IHC-P | (1) | 1/500 - 1/2500. |
| IHC-Fr | (1) | Use at an assay dependent concentration. |
| WB | 1/1000 - 1/5000. |
FI antibody
FITC antibody
fluorescein isothyocianate antibody
fluorescyl antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Fluorescein antibody (ab6656)This image is courtesy of an anonymous Abreview
Immunohistochemical analysis of Mouse thymus in which the medullary epithellai cells are labelled with fluorescein-conjugated UEA1 lectin with ab6656. Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 40 minutes at 20°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 20°C.
Immunohistochemistry (Frozen sections) - HRP Anti-Fluorescein antibody (ab6656)This image is courtesy of an anonymous Abreview
ab6656 staining GFP in Mouse mammary carcinoma metastasis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Perfusion fixed tissue with 4% paraformaldehyde, blocked with 0.5% Perkin-Elmer TNB blocking reagent for 30 minutes at 25°C and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500) for 30 minutes at 25°C
Micrograph demonstrates use of ab6662 for detection of GFP-positive tumor cells (LEFT) and an adjacent section after amplification with ab6656 (RIGHT). Images were taken with identical settings on a Leica confocal microscope.
