Anti-Collagen VI antibody

• 产品名称

  Anti-Collagen VI抗体
  参阅全部 Collagen VI 一抗

• 描述

  兔多克隆抗体to Collagen VI

• 宿主

  Rabbit

• 特异性

  Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat, Sheep, Rabbit, Dog, Pig, Mammals

• 免疫原

  Full length native protein (purified) corresponding to Human Collagen VI aa 1-1028. Collagen Type VI from human and bovine placenta.
  Database link: P12109

• 阳性对照

• WB: HepG2 whole cell lysate.

• 常规说明

  At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.2
  Preservative: 0.01% Sodium azide
  Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride

• 浓度

• 批次浓度范围 100 µg 浓度为 1 - 1.2 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  Anti-Collagen Type VI has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab6588于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 1291 Human

• Entrez Gene: 1292 Human

• Entrez Gene: 1293 Human

• Entrez Gene: 12833 Mouse

• Entrez Gene: 12834 Mouse

• Entrez Gene: 12835 Mouse

• Entrez Gene: 294337 Rat

• Entrez Gene: 361821 Rat

• Omim: 120220 Human

• Omim: 120240 Human

• Omim: 120250 Human

• SwissProt: P12109 Human

• SwissProt: P12110 Human

• SwissProt: P12111 Human

• SwissProt: Q02788 Mouse

• SwissProt: Q04857 Mouse

• Unigene: 474053 Human

• Unigene: 2509 Mouse

别名

• Alpha 1 (VI) chain (61 AA) antibody

• CO6A1_HUMAN antibody

• COL6A1 antibody

• COL6A2 antibody

• COL6A3 antibody

• collagen 6 antibody

• Collagen alpha 2(VI) chain antibody

• Collagen alpha 3(VI) chain antibody

• Collagen alpha-1(VI) chain antibody

• collagen six antibody

• Collagen type VI alpha 1 antibody

• Collagen type VI alpha 2 antibody

• Collagen type VI alpha 3 antibody

• Collagen VI alpha 1 polypeptide antibody

• Collagen VI alpha 2 polypeptide antibody

• Collagen VI alpha 3 polypeptide antibody

• CollagenVI antibody

• Human mRNA for collagen VI alpha 2 C terminal globular domain antibody

• OPLL antibody

• PP3610 antibody

Western blot - Anti-Collagen VI antibody (ab6588)

Anti-Collagen VI antibody (ab6588) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 108 kDa
Observed band size: 100 kDawhy is the actual band size different from the predicted?