生物素Anti-Fibronectin抗体
参阅全部 Fibronectin 一抗
生物素兔多克隆抗体to Fibronectin
Rabbit
Biotin
Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.
适用于: IHC-P, WBmore details
与反应: Human
预测可用于: Mammals
Full length native protein (purified) corresponding to Human Fibronectin. Purified from human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration.
Database link: P02751
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
Immunogen affinity purified
Anti-fibronectin (rabbit) antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificity.
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
多克隆
IgG
Abpromise™承诺保证使用ab6584于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/50 - 1/200. | |
| WB | 1/500 - 1/5000. |
Entrez Gene: 2335 Human
Omim: 135600 Human
SwissProt: P02751 Human
Unigene: 203717 Human
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FINC_HUMAN antibody
FN antibody
FN1 antibody
FNZ antibody
GFND antibody
GFND2 antibody
LETS antibody
Migration stimulating factor antibody
MSF antibody
Ugl-Y3 antibody
CIG antibody
Cold insoluble globulin antibody
Cold-insoluble globulin antibody
Western blot - Biotin Anti-Fibronectin antibody (ab6584)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fibronectin antibody (ab6584)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue sections at pH 6.0, labelling Fibronectin with ab6584 at a concentration of 10 µg/mL for 1 hour at room temperature. The secondary used was a rabbit peroxidase conjugate at a 1/10,000 dilution, incubated for 45 minutes at room temperature. Fibronectin is shown as a brown hue against a purple counterstain labelling nuclear DNA. The negative control on right (B) is without the primary antibody. Magnifications are as shown.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fibronectin antibody (ab6584)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue sections at pH 9.0, labelling Fibronectin with ab6584 at a concentration of 10 µg/mL for 1 hour at room temperature. The secondary used was a rabbit peroxidase conjugate at a 1/10,000 dilution, incubated for 45 minutes at room temperature. Fibronectin is shown as a brown hue against a purple counterstain labelling nuclear DNA. The negative control on right (B) is without the primary antibody. Magnifications are as shown.
