Anti-HSV1 + HSV2 gB抗体[10B7]
小鼠单克隆抗体[10B7] to HSV1 + HSV2 gB
Mouse
适用于: WB, ICC/IFmore details
与反应: Herpes simplex virus, Human herpesvirus 2
Tissue, cells or virus corresponding to HSV1 + HSV2 gB.
African Green Monkey Kidney cultured cells(Vero), HSV-1 is strain MacIntyre, HSV-2 is strain G.
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Liquid
Shipped at 4°C. Store at +4°C.
pH: 7.40
Constituent: 100% PBS
浓度
100 µg 浓度为 1 mg/ml
100 µg 浓度为 1 mg/ml
Protein A purified
单克隆
10B7
NS1/1-Ag4-1
IgG1
kappa
Abpromise™承诺保证使用ab6506于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | Use at an assay dependent concentration. 1/1600 (HSV2) - 1/12800 (HSV1). | |
ICC/IF | Use at an assay dependent concentration. 1/6400 (HSV1) - 1/25600 (HSV2). |
Entrez Gene: 1487312 Herpes simplex virus
Entrez Gene: 2703455 Herpes simplex virus
SwissProt: P08666 Herpes simplex virus
SwissProt: P10211 Herpes simplex virus
HSV1 antibody
HSV2 antibody
UL27 antibody
Envelope glycoprotein B antibody
GB antibody
Glycoprotein B antibody
Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506)
Green: immunofluorescence staining of HSV1 or HSV2 of Vero (African green monkey) cells ( HSV-1 is strain MacIntyre, HSV-2 is strain G) on slides, using ab6506 (1:100) (secondary anti-mouse-FITC). Red: counterstain in the conjugate preparation.
Western blot - Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506)
Lanes 1 & 8 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/1600 dilution
Lane 2 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/3200 dilution
Lane 3 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/6400 dilution
Lane 4 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/12800 dilution
Lane 5 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/200 dilution
Lane 6 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/400 dilution
Lane 7 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/800 dilution
Lanes 1-4 : Vero cells infected with HSV1
Lanes 5-8 : Vero cells infected with HSV2
Performed under reducing conditions.