Anti-Cathepsin L + V抗体[33/2]
参阅全部 Cathepsin L + V 一抗
小鼠单克隆抗体[33/2] to Cathepsin L + V
Mouse
Reacts specifically with the native and denatured forms of human cathepsin L (25kD) and procathepsin L (42 kD). Does not react with human cathepsin types B1, D1, H and S1, procathepsins B and D1 and recombinant procathepsin H, rat cathepsin B1, and mouse procathepsins B1 and D1.
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt
与反应: Mouse, Human
预测可用于: Rat
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
Recognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule).
WB: Human and Mouse lung tissue lysates. Human, Mouse tissue lysates. IHC-P: FFPE Human colon adenocarcinoma tissue sections.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team.
浓度
10 µg 浓度为 1 mg/ml
100 µg 浓度为 1 mg/ml
Affinity purified
单克隆
33/2
IgG1
Abpromise™承诺保证使用ab6314于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) | 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 38 kDa). Abcam recommends using 3% milk as the blocking agent. |
| IHC-P | (1) | Use at an assay dependent concentration. |
应用说明
Is unsuitable for Flow Cyt.
![Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)](https://www.abcam.cn/ps/products/6/ab6314/Images/ab6314-256349-anti-cathepsin-l-v-antibody-33-2-western-blot.jpg)
Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)
Lane 1 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20 dilution
Lane 2 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/100 dilution
Lane 3 : Anti-His tag at 1/10000 dilution
All lanes : Human Cathepsin L recombinant protein fraction
Secondary
Lanes 1 & 3 : Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/1000 dilution
Lane 2 : Rabbit anti-Mouse IgG at 1/2000 dilution
Predicted band size: 38 kDa
Blocking buffer and concentration:5% NFDM/TBST
Diluting buffer and concentration:5% NFDM/TBST
Observed MW:30
Exposure time:3 minutes
![Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)](https://www.abcam.cn/ps/products/6/ab6314/Images/ab6314-256350-anti-cathepsin-l-v-antibody-33-2-western-blot.jpg)
Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)
Lane 1 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20 dilution
Lane 2 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/100 dilution
Lane 3 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/500 dilution
Lane 4 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/100 dilution
Lane 5 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/1000 dilution
Lane 6 : Anti-His tag at 1/10000 dilution
All lanes : Human Cathepsin V recombinant protein fraction
Secondary
Lanes 1-3 : Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/1000 dilution
Lanes 4-5 : Rabbit Anti-Mouse secondary ab at 1/2000 dilution
Lane 6 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa
Blocking buffer and concentration:5% NFDM/TBST
Diluting buffer and concentration:5% NFDM/TBST
Observed MW:30
Exposure time:3 minutes
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L + V antibody [33/2] (ab6314)](https://www.abcam.cn/ps/products/6/ab6314/Images/ab6314-235240-anti-cathepsin-l-v-antibody-33-2-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L + V antibody [33/2] (ab6314)
IHC image of Cathepsin L staining in human colon adenocarcinoma formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6314, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
![Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)](https://www.abcam.cn/ps/products/6/ab6314/Images/ab6314-12236-anti-cathepsin-l-v-antibody-33-2-western-blot.jpg)
Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)
All lanes : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/1000 dilution (3% milk block)
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Lung (Mouse) Tissue Lysate
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : Kidney (Mouse) Tissue Lysate
Lane 5 : A549 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 25 kDawhy is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
![Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)](https://www.abcam.cn/ps/products/6/ab6314/Images/ab6314-12235-anti-cathepsin-l-v-antibody-33-2-western-blot.jpg)
Western blot - Anti-Cathepsin L + V antibody [33/2] (ab6314)Image courtesy of Ratnakar Singh by Abreview.
Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/750 dilution + whole cell lysate prepared from U87MG human glioblastoma at 50 µg
Secondary
Goat anti-mouse HRP at 1/2000 dilution
Predicted band size: 38 kDa
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