Anti-Collagen IV antibody [COL-94]

• 产品名称

  Anti-Collagen IV抗体[COL-94]
  参阅全部 Collagen IV 一抗

• 描述

  小鼠单克隆抗体[COL-94] to Collagen IV

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-Pmore details

• 种属反应性

  与反应: Human
  预测可用于: Non human primates不与反应: Rat, Sheep, Goat, Chicken, Cat

• 免疫原

  Full length native protein (purified) corresponding to Human Collagen IV.
  Database link: P02462

• 表位

  Recognises an epitope located on the alpha 1 and/or alpha 2 chains of human collage type IV.

• 阳性对照

• Natural Human Collagen IV protein (ab7536) can be used as a positive control in WB. Human skin tissue.

• 常规说明

  This product was changed from ascites to tissue culture supernatant on 10th July 2019. Lot numbers higher than GR3230927 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.1% Sodium azide
  Constituent: PBS

• 浓度

• 50 µl 浓度为 1 mg/ml

• 纯度

  Proprietary Purification

• 纯化说明

  Purified from hybridoma cell culture

• 克隆

  单克隆

• 克隆编号

  COL-94

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab6311于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 1282 Human

• Entrez Gene: 1284 Human

• Entrez Gene: 1285 Human

• Entrez Gene: 1286 Human

• Entrez Gene: 1287 Human

• Omim: 120070 Human

• Omim: 120090 Human

• Omim: 120130 Human

• Omim: 120131 Human

• Omim: 303630 Human

• SwissProt: P02462 Human

• SwissProt: P08572 Human

• SwissProt: P29400 Human

• SwissProt: P53420 Human

• SwissProt: Q01955 Human

• Unigene: 17441 Human

别名

• Arresten antibody

• BSVD antibody

• CO4A1_HUMAN antibody

• COL4A1 antibody

• COL4A1 NC1 domain antibody

• COL4A2 antibody

• COL4A3 antibody

• COL4A4 antibody

• COL4A5 antibody

• collagen alpha-1(IV) chain antibody

• Collagen IV Alpha 1 Polypeptide antibody

• Collagen IV Alpha 2 Polypeptide antibody

• Collagen Of Basement Membrane Alpha 1 Chain antibody

• Collagen Of Basement Membrane Alpha 2 Chain antibody

• Collagen Type IV Alpha 1 antibody

• collagen type IV alpha 1 chain antibody

• Collagen Type IV Alpha 2 antibody

• Collagen Type IV Alpha 3 antibody

• Collagen Type IV Alpha 4 antibody

• Collagen Type IV Alpha 5 antibody

• RATOR antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (ab6311)

  Immunohistochemical analysis of paraffin-embedded human tongue tissue staining Collagen IV with ab6311 at 8µg/ml. The antibody was developed using biotin/ExtrAvidin®-Peroxidase.

  This image was generated using the ascites version of the product.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [COL-94] (ab6311)This image is courtesy of an abreview submitted by Birgitta Weijdegard, Sahlgrenska Cancer Center.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovary tissue sections labeling Collagen IV with unpurified ab6311 at 1/400 dilution. Tissue was blocked with 1.4% serum for 30 minutes. Heat mediated antigen retrieval was performed. An undiluted monoclonal alkaline phosphatase conjugated secondary antibody was used.
  Blood vessels in whole ovarian section. Clear staining is seen in the vessel walls.

  This image was generated using the ascites version of the product.

  See Abreview