Anti-Tyrosine Hydroxylase抗体
参阅全部 Tyrosine Hydroxylase 一抗
兔多克隆抗体to Tyrosine Hydroxylase
Rabbit
Immunohistochemical distribution throughout the brain shows that staining is restricted to these neurons that are known to contain the enzyme.
适用于: IHC-FoFr, WB, ICC/IF, IHC-Frmore details
与反应: Mouse, Rat
预测可用于: Chicken, Chimpanzee, Macaque monkey
Synthetic peptide corresponding to Rat Tyrosine Hydroxylase aa 1-100 conjugated to keyhole limpet haemocyanin (Glutaraldehyde).
Database link: P04177
This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7
Constituent: Whole serum
Whole antiserum
This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.
多克隆
IgG
Abpromise™承诺保证使用ab6211于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. | |
| WB | 1/100 - 1/500. Predicted molecular weight: 42,53,84 kDa. | |
| ICC/IF | 1/1000. | |
| IHC-Fr | (2) | Use at an assay dependent concentration. |
Entrez Gene: 21823 Mouse
SwissProt: P24529 Mouse
Unigene: 1292 Mouse
Unigene: 11082 Rat
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Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody (ab6211)
ab6211 staining tyrosine hydroxylase in PC-12 cells treated with orexin A (ab120212), by ICC/IF. Decrease in expression of tyrosine hydroxylase correlates with increased concentration of orexin A, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120212 (orexin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum (ab7481), 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6211 (1/1000 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Western blot - Anti-Tyrosine Hydroxylase antibody (ab6211)
Anti-Tyrosine Hydroxylase antibody (ab6211) at 1/100 dilution + PC12 cell lysates at 6 µg
Performed under reducing conditions.
Predicted band size: 42,53,84 kDa
Three bands are detected at ≈ 42 kDa, 53 kDa and 84 kDa.
Immunohistochemistry (Frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)Image courtesy of QBM Cell Sciences, Ottawa, Canada
ab6211 Rabbit polyclonal to Tyrosine Hydroxylase (1/1000) on cryopreserved ventral mesencephalic neurons dissected at mouse embryonic day 14. Neurons were thawed, plated and grown for 14 days, before fixation and overnight staining with ab6211.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tyrosine Hydroxylase antibody (ab6211)
Immunohistochemical detection of dopaminergic neurons in the rat zona incerta in a formalin-fixed floated cryostat section. Tyrosine hydroxylase immunoreactivity was visualized with ab6211 (1:100,000), using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.
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