Anti-NGF抗体- BSA and Azide free
参阅全部 NGF 一抗
兔多克隆抗体to NGF - BSA and Azide free
Rabbit
Less than 1% cross-reactivity against recombinant human Brain Derived Neurotrophic Factor, Neurotrophin 3 and Neurotrophin 4/5 by ELISA.
适用于: ICC/IF, WBmore details
与反应: Mouse, Human
不与反应: Cow
Full length native protein (purified) corresponding to Mouse NGF.
Database link: P01139
This antibody has been shown to be useful for a variety of techniques and its specificity has been demonstrated by immunoblot.
Before storing ab6199 long-term, please follow the aliquot instructions.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7
Constituent: PBS
pH: 7.2-7.6 without preservatives.
是
Protein G purified
多克隆
IgG
Abpromise™承诺保证使用ab6199于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) | Use a concentration of 10 µg/ml. |
| WB | (2) | Use a concentration of 1 µg/ml. |
Entrez Gene: 4803 Human
Entrez Gene: 18049 Mouse
Omim: 162030 Human
SwissProt: P01138 Human
SwissProt: P01139 Mouse
Unigene: 2561 Human
Unigene: 1259 Mouse
Beta-NGF antibody
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Nerve growth factor beta polypeptide antibody
Nerve growth factor beta subunit antibody
NGF antibody
NGF_HUMAN antibody
NGFB antibody
NID67 antibody
Beta nerve growth factor antibody
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Western blot - Anti-NGF antibody - BSA and Azide free (ab6199)
All lanes : Anti-NGF antibody - BSA and Azide free (ab6199) at 5 µg/ml
Lane 1 : rhNGF protein at 100 µg
Lane 2 : Mouse salivary gland tissue lysate at 50 µg
ab6199 detects a strong band at 13 kDa consistent with the expected molecular weight of mature NGF monomer.
Western blot - Anti-NGF antibody - BSA and Azide free (ab6199)
Anti-NGF antibody - BSA and Azide free (ab6199) at 1 µg/ml + Active human NGF full length protein (ab69759) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 seconds
Immunocytochemistry/ Immunofluorescence - Anti-NGF antibody - BSA and Azide free (ab6199)
ICC/IF image of ab6199 stained MEF1 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6199, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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