Anti-pan Synuclein抗体
参阅全部 pan Synuclein 一抗
兔多克隆抗体to pan Synuclein
Rabbit
This antibody recognises all 3 forms of the synucleins.
适用于: WB, IHC-P, IHC-Fr, ICC/IFmore details
与反应: Rat, Human
预测可用于: Mouse, Chicken, Cow, Xenopus laevis
Synthetic peptide corresponding to Human pan Synuclein aa 1-100. The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide (MSC) and coupled to diphtheria toxoid.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab6176于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | Use at an assay dependent concentration. |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | (1) | Use a concentration of 1 µg/ml. |
Entrez Gene: 6620 Human
Entrez Gene: 6622 Human
Entrez Gene: 6623 Human
Entrez Gene: 104069 Mouse
Entrez Gene: 20617 Mouse
Entrez Gene: 20618 Mouse
Omim: 163890 Human
Omim: 602569 Human
Omim: 602998 Human
SwissProt: O76070 Human
SwissProt: P37840 Human
SwissProt: Q16143 Human
SwissProt: Q6FHG5 Human
SwissProt: O55042 Mouse
SwissProt: Q91ZZ3 Mouse
SwissProt: Q9Z0F7 Mouse
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Immunocytochemistry/ Immunofluorescence - Anti-pan Synuclein antibody (ab6176)
ICC/IF image of ab6176 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6176, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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