Anti-p75 NGF Receptor抗体[MC-192]
参阅全部 p75 NGF Receptor 一抗
小鼠单克隆抗体[MC-192] to p75 NGF Receptor
Mouse
适用于: ICCmore details
与反应: Rat
Tissue, cells or virus corresponding to Rat p75 NGF Receptor. PC-12 cells.
This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC12.
A well characterised and versatile monoclonal antibody, clone number MC-192, that is of central importance in the study of LANGF (p75-NGF) receptor.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7
Constituent: PBS
Protein A purified
A well characterised and versatile monoclonal antibody, clone number MC-192, that is of central importance in the study of LANGF (p75-NGF) receptor.
单克隆
MC-192
IgG1
Abpromise™承诺保证使用ab6172于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC | Use at an assay dependent concentration. |
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Immunocytochemistry - Anti-p75 NGF Receptor antibody [MC-192] (ab6172)
ab6172 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab6172 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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