Anti-PABP antibody [10E10]

• 产品名称

  Anti-PABP抗体[10E10]
  参阅全部 PABP 一抗

• 描述

  小鼠单克隆抗体[10E10] to PABP

• 宿主

  Mouse

• 经测试应用

  适用于: IP, ICC/IF, WB, Flow Cytmore details

• 种属反应性

  与反应: Human
  不与反应: Mouse, Drosophila melanogaster

• 免疫原

  Recombinant PABP (Human) expressed from its 1.85 kbp cDNA, NcoI to SspI.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.1% Sodium azide
  Constituent: PBS

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Purified from supernatant.

• 克隆

  单克隆

• 克隆编号

  10E10

• 骨髓瘤

  Sp2/0

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab6125于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 26986 Human

• Omim: 604679 Human

• SwissProt: P11940 Human

• Unigene: 387804 Human

• 别名

• PABP-1 antibody

• PABP1 antibody

• PABP1_HUMAN antibody

• PABPC 1 antibody

• Pabpc1 antibody

• PABPC2 antibody

• PABPL1 antibody

• Poly A binding protein 1 antibody

• Poly A binding protein cytoplasmic 1 antibody

• poly(A) binding protein, cytoplasmic 1 antibody

• poly(A) binding protein, cytoplasmic 2 antibody

• Poly(A)-binding protein 1 antibody

• Polyadenylate binding protein 1 antibody

• Polyadenylate-binding protein 1 antibody

• PAB 1 antibody

• PAB1 antibody

• PABP 1 antibody

• 

  Immunoprecipitation - Anti-PABP antibody [10E10] (ab6125)

  PABP was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to PABP (ab6125) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
  The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
  Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab6125.
  Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
  Band: 76kDa: PABP; 25kDa.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-PABP antibody [10E10] (ab6125)

  ICC/IF image of ab6125 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6125, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

• 

  Flow Cytometry - Anti-PABP antibody [10E10] (ab6125)

  Overlay histogram showing HeLa cells stained with ab6125 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6125, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
  

• 

  Western blot - Anti-PABP antibody [10E10] (ab6125)

  Western Blot analysis on HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate using ab6125 at 1/100 to 1/6400 dilution. Anti-mouse IgG (whole molecule)-AP conjugate was used at the secondary at a 1/2000 dilution. Detection with BCIP/NBT substrate.
  4-12% Bis-Tris, 1X MOPS running buffer.