Anti-KPNA3抗体
参阅全部 KPNA3 一抗
山羊多克隆抗体to KPNA3
Goat
适用于: Flow Cyt, ICC/IF, WBmore details
与反应: Human
预测可用于: Dog
Synthetic peptide: DPTANLQTKEFNF, corresponding to C terminal amino acids 509-521 of Human KPNA3.
WB: CaCo-2 cell lysate, MCF-7 cell lysate, HEK293 cell lysate. Flow Cyt: A431 cells ICC/IF: A431 cells
GenBank Accession Number – NP_002258.
The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA3, encodes a protein similar to certain nuclear transport proteins of Xenopus and human. The predicted amino acid sequence shows similarity to Xenopus importin, yeast SRP1, and human RCH1 (KPNA2), respectively. The similarities among these proteins suggests that karyopherin alpha-3 may be involved in the nuclear transport system.
多克隆
IgG
Abpromise™承诺保证使用ab6038于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use a concentration of 10 µg/ml. | |
| ICC/IF | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 58 kDa). A 1 hour primary incubation is recommended for this product. |
Entrez Gene: 3839 Human
Omim: 601892 Human
SwissProt: O00505 Human
Unigene: 527919 Human
Importin alpha 3 antibody
Importin alpha 3 subunit antibody
Importin alpha 4 antibody
Importin alpha Q2 antibody
Importin subunit alpha-4 antibody
IPOA4 antibody
Karyopherin alpha 3 antibody
Karyopherin alpha 3 importin alpha 4 antibody
Karyopherin alpha 3 subunit antibody
Karyopherin subunit alpha 3 antibody
Karyopherin subunit alpha-3 antibody
KPNA 3 antibody
Kpna3 antibody
Qip2 antibody
SRP1 antibody
SRP1-gamma antibody
SRP1gamma antibody
SRP4 antibody
SRP1 gamma antibody
hSRP1 antibody
IMA4_HUMAN antibody
Flow Cytometry - Anti-KPNA3 antibody (ab6038)
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western blot - Anti-KPNA3 antibody (ab6038)
Lanes 1-2 : Anti-KPNA3 antibody (ab6038) at 0.03 µg/ml
Lane 3 : Anti-KPNA3 antibody (ab6038) at 0.01 µg/ml
Lane 1 : CaCo-2 cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : MCF-7 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 58 kDa
Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence - Anti-KPNA3 antibody (ab6038)
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
